Brassinosteroids regulate root growth by controlling reactive oxygen species homeostasis and dual effect on ethylene synthesis in Arabidopsis.
Brassinosteroids regulate root growth by controlling reactive oxygen species homeostasis and dual effect on ethylene synthesis in Arabidopsis.
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油菜素类固醇通过控制活性氧稳态和对拟南芥乙烯合成的双重作用来调节根系生长
DOI：
10.1371/journal.pgen.1007144
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发表时间：
2018-01
期刊：
影响因子：
4.5
通讯作者：
Ding Z
中科院分区：
文献类型：
--
作者：
Lv B;Tian H;Zhang F;Liu J;Lu S;Bai M;Li C;Ding Z
油菜素类固醇（BR）代表一类植物激素，其调节生长和发育的许多方面。在这里，det 2 -9突变体缺陷BR合成的EMS突变体筛选缺陷的根长度，并被用来调查BR在拟南芥根发育中的作用。det 2 -9突变体表现出短根表型，这是由于根分生组织细胞数量减少和根成熟区细胞尺寸减小所致。与野生型相比，det 2 -9突变体的乙烯合成大幅增加，导致乙烯过度积累，从而抑制根系生长。det 2 -9的短根表型在det 2 -9/acs 9双突变体和det 2 -9/ein 3/eil 1 -1三突变体中得到部分恢复，这两个突变体分别在乙烯合成或乙烯信号转导方面存在缺陷。外源BR的应用表明，BR或正或负调节乙烯生物合成的浓度依赖性的方式。与BR通过稳定ACSs的稳定性来诱导乙烯生物合成不同，BR信号转录因子BES 1和BZR 1直接与ACS 7、ACS 9和ACS 11的启动子相互作用，抑制其表达，表明BR在生理水平下存在天然调控机制。此外，与野生型对照相比，det 2 -9突变体表现出过量积累的超氧阴离子（O2-），且O2-水平的增加有助于根系生长的抑制。BR调控的O2-积累的控制作用通过过氧化物酶途径，而不是通过NADPH氧化酶途径。本研究揭示了BRs与乙烯或/和ROS之间的激素交叉调节参与拟南芥根生长发育的重要机制。油菜素类固醇（BR）和乙烯都被认为是控制根的生长和发育。ROS也被报道在根的发育中起重要作用。然而，BRs与乙烯或ROS在根生长发育中的关系以前没有得到解决。在本研究中，det 2 -9缺陷BR合成突变体鉴定从EMS突变体筛选，显示短根表型，这是由于超积累的乙烯和超氧阴离子（O2-）。外源BR对乙烯生物合成的调节作用呈浓度依赖性。与BR通过稳定ACS的稳定性来诱导乙烯生物合成不同，BR信号转录因子BES 1和BZR 1与ACS 7、ACS 9和ACS 11的启动子相互作用抑制其表达，表明BR在生理水平下存在天然调控机制。BR调控的O2-积累的控制作用通过过氧化物酶途径，而不是通过NADPH氧化酶途径。本研究为油菜素内酯如何通过与乙烯合成和活性氧的交叉调节来控制根系生长提供了新的见解。
The brassinosteroids (BRs) represent a class of phytohormones, which regulate numerous aspects of growth and development. Here, a det2-9 mutant defective in BR synthesis was identified from an EMS mutant screening for defects in root length, and was used to investigate the role of BR in root development in Arabidopsis. The det2-9 mutant displays a short-root phenotype, which is result from the reduced cell number in root meristem and decreased cell size in root maturation zone. Ethylene synthesis is highly increased in the det2-9 mutant compared with the wild type, resulting in the hyper-accumulation of ethylene and the consequent inhibition of root growth. The short-root phenotype of det2-9 was partially recovered in the det2-9/acs9 double mutant and det2-9/ein3/eil1-1 triple mutant which have defects either in ethylene synthesis or ethylene signaling, respectively. Exogenous application of BR showed that BRs either positively or negatively regulate ethylene biosynthesis in a concentration-dependent manner. Different from the BR induced ethylene biosynthesis through stabilizing ACSs stability, we found that the BR signaling transcription factors BES1 and BZR1 directly interacted with the promoters of ACS7, ACS9 and ACS11 to repress their expression, indicating a native regulation mechanism under physiological levels of BR. In addition, the det2-9 mutant displayed over accumulated superoxide anions (O2-) compared with the wild-type control, and the increased O2- level was shown to contribute to the inhibition of root growth. The BR-modulated control over the accumulation of O2- acted via the peroxidase pathway rather than via the NADPH oxidase pathway. This study reveals an important mechanism by which the hormone cross-regulation between BRs and ethylene or/and ROS is involved in controlling root growth and development in Arabidopsis. Both brassinosteroids (BRs) and ethylene have been known to control root growth and development. ROS have been also reported to play an important role in root development. However, the relationship between BRs and ethylene or ROS in root growth and development was not addressed before. In this study, a det2-9 mutant defective in BR synthesis was identified from an EMS mutant screening, displaying a short-root phenotype which is result from the hyper-accumulation of ethylene and superoxide anions (O2-). Exogenous BR apply showed that BRs either positively or negatively regulate ethylene biosynthesis in a concentration-dependent manner. Different from the BR induced ethylene biosynthesis through stabilizing ACSs stability, we found that the BR signaling transcription factors BES1 and BZR1 interacted with promoters of ACS7, ACS9 and ACS11 to repress their expression, indicating a native regulation mechanism under physiological levels of BR. The BR-modulated control over the accumulation of O2- acted via the peroxidase pathway rather than via the NADPH oxidase pathway. This study provides new insights into how brassinosteroids control root growth through the cross-regulation with ethylene synthesis and ROS.
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黄铜固醇，生长素和细胞分裂素对拟南芥植物乙烯生产的影响。
影响因子：
6.9
作者：
Arteca, Richard N.;Arteca, Jeannette M.
通讯作者：
Arteca, Jeannette M.
影响因子：
7.2
作者：
Colón-Carmona, A;You, R;Doerner, P
通讯作者：
Doerner, P
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
11.6
作者：
Choe, SW;Noguchi, T;Feldmann, KA
通讯作者：
Feldmann, KA
DOI：
10.1111/j.1365-313x.2008.03711.x
发表时间：
2009-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Hansen M;Chae HS;Kieber JJ
通讯作者：
Kieber JJ