Confined Sandwichlike Microenvironments Tune Myogenic Differentiation.
Confined Sandwichlike Microenvironments Tune Myogenic Differentiation.
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DOI：
10.1021/acsbiomaterials.7b00109
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发表时间：
2017-08-14
影响因子：
5.8
通讯作者：
Salmerón-Sánchez M
中科院分区：
文献类型：
--
作者：
Ballester-Beltrán J;Trujillo S;Alakpa EV;Compañ V;Gavara R;Meek D;West CC;Péault B;Dalby MJ;Salmerón-Sánchez M
三明治状(SW)培养是一种多层技术，可以同时刺激背侧和腹侧细胞受体，寻求以简化的方式模拟三维(3D)环境中的细胞黏附。研究了这种环境对在标准生长介质中培养的几种细胞类型的细胞分化的影响，标准生长介质促进了二维(2D)表面的增殖，避免了任何优先分化。首先，小鼠C2C12成肌细胞表现出特异性的成肌分化。脂肪和骨髓来源的人骨髓间充质干细胞(HMSCs)可以向更广泛的谱系分化，再次显示出肌肉分化。总体而言，这项研究显示了在西南条件下，几种细胞类型在正常生长介质中的肌源性分化，避免使用生长因子和细胞因子，即仅通过在西南环境中培养细胞。在力学上，它提供了进一步的洞察力，以了解整合素与背部底物之间的黏附和由短波系统施加的限制之间的平衡。
Sandwichlike (SW) cultures are engineered as a multilayer technology to simultaneously stimulate dorsal and ventral cell receptors, seeking to mimic cell adhesion in three-dimensional (3D) environments in a reductionist manner. The effect of this environment on cell differentiation was investigated for several cell types cultured in standard growth media, which promotes proliferation on two-dimensional (2D) surfaces and avoids any preferential differentiation. First, murine C2C12 myoblasts showed specific myogenic differentiation. Human mesenchymal stem cells (hMSCs) of adipose and bone marrow origin, which can differentiate toward a wider variety of lineages, showed again myodifferentiation. Overall, this study shows myogenic differentiation in normal growth media for several cell types under SW conditions, avoiding the use of growth factors and cytokines, i.e., solely by culturing cells within the SW environment. Mechanistically, it provides further insights into the balance between integrin adhesion to the dorsal substrate and the confinement imposed by the SW system.
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