The "8-kD" cytoplasmic dynein light chain is required for nuclear migration and for dynein heavy chain localization in Aspergillus nidulans.
The "8-kD" cytoplasmic dynein light chain is required for nuclear migration and for dynein heavy chain localization in Aspergillus nidulans.
复制标题
DOI：
10.1083/jcb.143.5.1239
复制
发表时间：
1998-11-30
期刊：
影响因子：
--
通讯作者：
Ronald Morris N
中科院分区：
文献类型：
--
作者：
Beckwith SM;Roghi CH;Liu B;Ronald Morris N
胞质动力蛋白的重链是曲霉和其他真菌进行核迁移所必需的。在这里，我们报道了一个新的核迁移所需的基因nudG，它编码“8-kD”细胞质动力蛋白轻链(CDLC)的同源物。我们证明了温度敏感的nudG8突变在有限的温度下抑制了核的迁移和生长。该突变还抑制了无性和有性孢子形成，降低了nudG CDLC蛋白的细胞内浓度，并导致通常位于野生型菌丝中的细胞质动力蛋白重链在菌丝顶端缺失。抗胞浆动力蛋白重链(CDHC)抗体和nudG CDLC抗体的免疫共沉淀实验表明，胞质动力蛋白轻链的一部分与CDHC形成蛋白质复合体。然而，蔗糖梯度沉淀分析表明，并不是所有的NUDG蛋白都与重链络合。携带细胞质动力蛋白重链缺失和温度敏感的nudG突变的双突变株在限制性温度下的生长速度并不比只有CDHC缺失的菌株慢。这一结果表明，nudG突变对核迁移和生长的影响是通过与CDHC相互作用而不是与8-kD CDLC理论上可能与之相互作用的其他分子(如肌球蛋白-V)介导的。
The heavy chain of cytoplasmic dynein is required for nuclear migration in Aspergillus nidulans and other fungi. Here we report on a new gene required for nuclear migration, nudG, which encodes a homologue of the “8-kD” cytoplasmic dynein light chain (CDLC). We demonstrate that the temperature sensitive nudG8 mutation inhibits nuclear migration and growth at restrictive temperature. This mutation also inhibits asexual and sexual sporulation, decreases the intracellular concentration of the nudG CDLC protein and causes the cytoplasmic dynein heavy chain to be absent from the mycelial tip, where it is normally located in wild-type mycelia. Coimmunoprecipitation experiments with antibodies against the cytoplasmic dynein heavy chain (CDHC) and the nudG CDLC demonstrated that some fraction of the cytoplasmic dynein light chain is in a protein complex with the CDHC. Sucrose gradient sedimentation analysis, however, showed that not all of the NUDG protein is complexed with the heavy chain. A double mutant carrying a cytoplasmic dynein heavy chain deletion plus a temperature-sensitive nudG mutation grew no more slowly at restrictive temperature than a strain with only the CDHC deletion. This result demonstrates that the effect of the nudG mutation on nuclear migration and growth is mediated through an interaction with the CDHC rather than with some other molecule (e.g., myosin-V) with which the 8-kD CDLC might theoretically interact.
登录
查看更多内容
影响因子：
14.9
作者：
BRODY, H;GRIFFITH, J;TIMBERLAKE, WE
通讯作者：
TIMBERLAKE, WE
DOI：
10.1083/jcb.138.3.629
发表时间：
1997-08-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carminati JL;Stearns T
通讯作者：
Stearns T
影响因子：
64.8
作者：
HATTORI, M;ADACHI, H;INOUE, K
通讯作者：
INOUE, K
影响因子：
3.3
作者：
CLARK, SW;STAUB, O;MEYER, DI
通讯作者：
MEYER, DI
DOI：
10.1083/jcb.122.1.113
发表时间：
1993-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Baker J;Theurkauf WE;Schubiger G
通讯作者：
Schubiger G