Molecular characterization of a short peptidoglycan recognition protein (PGRP-S) from Asian corn borer (Ostrinia furnacalis) and its role in triggering proPO activity
Molecular characterization of a short peptidoglycan recognition protein (PGRP-S) from Asian corn borer (Ostrinia furnacalis) and its role in triggering proPO activity
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亚洲玉米螟 (Ostriniafurnacalis) 短肽聚糖识别蛋白 (PGRP-S) 的分子特征及其在触发 proPO 活性中的作用
DOI：
10.1007/s11274-013-1449-3
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发表时间：
2013-08
影响因子：
4.1
通讯作者：
Feng-Liang jin
中科院分区：
文献类型：
--
作者：
Qiang Sun;Xiao-Xia Xu;Shoaib Freed;Wan-Jun Huang;Feng-Liang jin
肽聚糖识别蛋白（PGRP）是昆虫、脊椎动物等的非特异性免疫分子，但不存在于植物和线虫中。本实验从亚洲玉米螟基因组文库中克隆了一个PGRP基因（2，910 bp，含4个外显子），并克隆了一个PGRP全长cDNA（设计为OfPGRP-S），开放阅读框为579 bp，含192个氨基酸。该推测的氨基酸序列与已知的鳞翅目PGRP显示出最大的相似性。实时荧光定量PCR检测结果表明，OfPGRP-S基因在整个发育阶段均组成型表达，且在成熟幼虫中表达量较高。甚至OfPGRP-S主要在具有免疫能力的器官中表达，脂肪体和中肠，并强烈诱导注射革兰氏阳性细菌，即，金黄色葡萄球菌。重组蛋白OfPGRP-S能与S结合。aureus和苏云金芽孢杆菌，它们在这些微生物存在下增强proPO活化。结果表明，OfPGRP-S是一种诱导蛋白，作为受体型PGRP，用于增强暴露于细菌时的proPO活化。
Peptidoglycan recognition proteins (PGRPs) are non-specific immune molecules of insects, and vertebrates etc., but are not present in plants and nematodes. In the current experiment, a PGRP DNA sequence (2,910 bp containing four exons) was identified from genomic DNA library of Asian corn borer,Ostrinia furnacalis, and a full-length cDNA programming PGRP was cloned (designed as OfPGRP-S) with an open reading frame of 579 bp, having 192 amino acid. This inferred amino acid sequence showed maximum similarity to known lepidopteran PGRPs. Quantitative real-time PCR investigation disclosed the level of mRNA of OfPGRP-S to be constitutively expressed in the whole developmental stages and with higher expression in the mature larvae. Even more the OfPGRP-S was mainly expressed in immune capable organs i.e., fat body and midgut, and was strongly induced by injecting gram-positive bacteria i.e.,Staphylococus aureus. Recombinant protein OfPGRP-S could bind toS. aureusandBacillus thuringiensiswhich enhance proPO activation in the presence of these microbes. The results indicated that OfPGRP-S is an inducible protein acting as a receptor-type PGRP for enhancing the proPO activation on exposure to bacteria.
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DOI：
10.1074/jbc.271.23.13854
发表时间：
1996-06
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
H. Yoshida;Kuninori Kinoshita;M. Ashida
通讯作者：
H. Yoshida;Kuninori Kinoshita;M. Ashida
DOI：
10.1073/pnas.97.25.13772
发表时间：
2000-12-05
影响因子：
11.1
作者：
Werner, T;Liu, G;Hultmark, D
通讯作者：
Hultmark, D
影响因子：
30.5
作者：
Kaneko, Takashi;Yano, Tamaki;Silverman, Neal
通讯作者：
Silverman, Neal
影响因子：
2.9
作者：
H. You;Hu Wan;Jianhong Li;B. Jin
通讯作者：
H. You;Hu Wan;Jianhong Li;B. Jin
DOI：
10.1016/j.cbpb.2007.03.005
发表时间：
2007-07-01
影响因子：
2.2
作者：
Onoe, Hiroko;Matsumoto, Akiyoshi;Morishima, Isao
通讯作者：
Morishima, Isao