Current state of diagnostic, screening and surveillance testing methods for COVID-19 from an analytical chemistry point of view.
Current state of diagnostic, screening and surveillance testing methods for COVID-19 from an analytical chemistry point of view.
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DOI：
10.1016/j.microc.2021.106305
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Asuero AG
中科院分区：
文献类型：
--
作者：
Martín J;Tena N;Asuero AG
自 2019 年 12 月以来，我们一直在应对人类面临的新威胁，即严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2)。在这篇综述中，我们描述了用于诊断、筛查和/或监测 SARS-CoV-2 的四种主要方法： 实时逆转录聚合酶链反应 (RT-PCR)；胸部计算机断层扫描（CT）；并开发了不同的补充替代方案，以获得快速结果、抗原和抗体检测。他们都从分析的角度比较了突出的优点和缺点。就灵敏度和特异性而言，金标准方法是 RT-PCR。还提出并讨论了旨在使其在护理点 (POC) 更加快速和适用的不同修改。 CT图像仅限于中心医院。然而，与 RT-PCR 相结合是确认 COVID-19 感染的最可靠、最准确的方法。抗体检测虽然无法提供有关感染状况的可靠结果，但适合对人群进行最大程度的筛查，以了解免疫能力。最近，抗原检测的灵敏度不如 RT-PCR，但已被授权在分析时更快地确定患者是否受到感染，且无需特定仪器。
Since December 2019, we have been in the battlefield with a new threat to the humanity known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this review, we describe the four main methods used for diagnosis, screening and/or surveillance of SARS-CoV-2: Real-time reverse transcription polymerase chain reaction (RT-PCR); chest computed tomography (CT); and different complementary alternatives developed in order to obtain rapid results, antigen and antibody detection. All of them compare the highlighting advantages and disadvantages from an analytical point of view. The gold standard method in terms of sensitivity and specificity is the RT-PCR. The different modifications propose to make it more rapid and applicable at point of care (POC) are also presented and discussed. CT images are limited to central hospitals. However, being combined with RT-PCR is the most robust and accurate way to confirm COVID-19 infection. Antibody tests, although unable to provide reliable results on the status of the infection, are suitable for carrying out maximum screening of the population in order to know the immune capacity. More recently, antigen tests, less sensitive than RT-PCR, have been authorized to determine in a quicker way whether the patient is infected at the time of analysis and without the need of specific instruments.
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DOI：
10.1016/j.jiac.2020.11.021
发表时间：
2021-04
期刊：
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy
影响因子：
--
作者：
Aoki K;Nagasawa T;Ishii Y;Yagi S;Okuma S;Kashiwagi K;Maeda T;Miyazaki T;Yoshizawa S;Tateda K
通讯作者：
Tateda K
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影响因子：
4.3
作者：
Benzigar MR;Bhattacharjee R;Baharfar M;Liu G
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Liu G
影响因子：
6.1
作者：
Abid SA;Ahmed Muneer A;Al-Kadmy IMS;Sattar AA;Beshbishy AM;Batiha GE;Hetta HF
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Hetta HF
影响因子：
6.4
作者：
Cai, Xue-fei;Chen, Juan;Wang, De-qiang
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Wang, De-qiang
影响因子：
8.8
作者：
Bordi, Licia;Piralla, Antonio;Capobianchi, Maria Rosaria
通讯作者：
Capobianchi, Maria Rosaria