Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia.
Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia.
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丹芪丸对心肌缺血整体基因表达变化的多靶点作用
DOI：
10.1155/2018/9469670
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发表时间：
2018
影响因子：
2.9
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Wang Q;Meng H;Zhang Q;Shi T;Zhang X;Shao M;Lu L;Wang J;Wang W;Li C;Wang Y
丹芪丸（DQP）是一种广泛用于治疗心血管疾病的中药（TCM）。本研究的目的是系统地表征大鼠模型中心肌缺血 (MI) 引起的基因表达模式改变，并研究 DQP 对整体基因表达的影响。测量了总体 mRNA 表达。分析假手术组、模型组和DQP组之间的差异表达基因。对差异表达基因进行基因本体富集分析和通路分析。我们量化了 10,813 个基因。与假手术组相比，模型组有339个基因表达上调，177个基因表达下调。上调的基因在细胞外基质组织、受伤反应和防御反应途径中富集。下调基因富集于脂肪酸代谢、丙酮酸代谢、PPAR信号通路等。这表明心肌梗死大鼠出现了能量代谢紊乱。在 DQP 组中，改变途径中的基因表达被调节回到正常水平。 DQP逆转了模型组中516个差异表达基因中的313个的表达。这项研究深入了解了中医治疗复杂疾病的多靶点机制。
Danqi pill (DQP) is a widely prescribed traditional Chinese medicine (TCM) in the treatment of cardiovascular diseases. The objective of this study is to systematically characterize altered gene expression pattern induced by myocardial ischemia (MI) in a rat model and to investigate the effects of DQP on global gene expression. Global mRNA expression was measured. Differentially expressed genes among the sham group, model group, and DQP group were analyzed. The gene ontology enrichment analysis and pathway analysis of differentially expressed genes were carried out. We quantified 10,813 genes. Compared with the sham group, expressions of 339 genes were upregulated and 177 genes were downregulated in the model group. The upregulated genes were enriched in extracellular matrix organization, response to wounding, and defense response pathways. Downregulated genes were enriched in fatty acid metabolism, pyruvate metabolism, PPAR signaling pathways, and so forth. This indicated that energy metabolic disorders occurred in rats with MI. In the DQP group, expressions of genes in the altered pathways were regulated back towards normal levels. DQP reversed expression of 313 of the 516 differentially expressed genes in the model group. This study provides insight into the multitarget mechanism of TCM in the treatment of complex diseases.
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DOI：
10.1152/ajpheart.00447.2006
发表时间：
2007-06-01
影响因子：
4.8
作者：
Brower, Gregory L.;Levick, Scott P.;Janicki, Joseph S.
通讯作者：
Janicki, Joseph S.
影响因子：
4.4
作者：
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Lemke N
DOI：
10.1016/j.jpba.2005.10.023
发表时间：
2006-04-11
影响因子：
3.4
作者：
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Wang, YT
影响因子：
5.4
作者：
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Lin, Shuguang
影响因子：
6.5
作者：
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通讯作者：
Schaffer, JE