Development of a Phage Cocktail to Target Salmonella Strains Associated with Swine.
Development of a Phage Cocktail to Target Salmonella Strains Associated with Swine.
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DOI：
10.3390/ph15010058
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发表时间：
2022-01-02
期刊：
影响因子：
--
通讯作者：
Clokie MRJ
中科院分区：
文献类型：
--
作者：
Thanki AM;Clavijo V;Healy K;Wilkinson RC;Sicheritz-Pontén T;Millard AD;Clokie MRJ
由多重耐药沙门氏菌菌株引起的感染在猪中是有问题的，并且正在进入人类食物链。噬菌体（Bacteriophages）可用于补充或替代抗生素，以减少猪体内的感染。在这里，我们广泛地表征了六种广泛宿主范围的裂解性沙门氏菌，目的是开发一种噬菌体鸡尾酒来预防或治疗感染。有趣的是，受试者之间存在一到五个单核苷酸多态性。然而，它们之间存在明显的表型差异，特别是在它们的热敏感性和pH敏感性方面。进行了体外杀灭试验，以确定单独使用和联合使用时的疗效，三种混合物在两小时内将细菌数量减少了约2 × 103 CFU/mL。在幼虫攻击研究中测试这些混合物，并且用噬菌体混合物SPFM 10-SPFM 14进行预防性处理是最有效的。噬菌体治疗改善幼虫存活率为90%，72小时后，在感染的未处理组为3%。在65%的噬菌体处理的幼虫，沙门氏菌计数低于检测限，而它是从100%的感染，未处理的幼虫组分离。这项研究表明，益生菌有效地减少了沙门氏菌在幼虫中的定植，这支持了它们类似地保护猪的能力。
Infections caused by multidrug resistant Salmonella strains are problematic in swine and are entering human food chains. Bacteriophages (phages) could be used to complement or replace antibiotics to reduce infection within swine. Here, we extensively characterised six broad host range lytic Salmonella phages, with the aim of developing a phage cocktail to prevent or treat infection. Intriguingly, the phages tested differed by one to five single nucleotide polymorphisms. However, there were clear phenotypic differences between them, especially in their heat and pH sensitivity. In vitro killing assays were conducted to determine the efficacy of phages alone and when combined, and three cocktails reduced bacterial numbers by ~2 × 103 CFU/mL within two hours. These cocktails were tested in larvae challenge studies, and prophylactic treatment with phage cocktail SPFM10-SPFM14 was the most efficient. Phage treatment improved larvae survival to 90% after 72 h versus 3% in the infected untreated group. In 65% of the phage-treated larvae, Salmonella counts were below the detection limit, whereas it was isolated from 100% of the infected, untreated larvae group. This study demonstrates that phages effectively reduce Salmonella colonisation in larvae, which supports their ability to similarly protect swine.
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影响因子：
5.2
作者：
Cools, Freya;Torfs, Eveline;Cos, Paul
通讯作者：
Cos, Paul
DOI：
10.1016/j.ejpb.2017.09.002
发表时间：
2017-12
期刊：
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
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DOI：
10.1016/j.ijfoodmicro.2011.08.015
发表时间：
2011-12-02
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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