Cytoprotective effect of short-term pretreatment with proanthocyanidin on human gingival fibroblasts exposed to harsh environmental conditions.
Cytoprotective effect of short-term pretreatment with proanthocyanidin on human gingival fibroblasts exposed to harsh environmental conditions.
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短期预处理对原藻苷素的细胞保护作用对暴露于恶劣环境条件的人牙龈成纤维细胞的细胞保护作用。
DOI：
10.1371/journal.pone.0113403
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Sasaki K
中科院分区：
文献类型：
--
作者：
Kurauchi M;Niwano Y;Shirato M;Kanno T;Nakamura K;Egusa H;Sasaki K
我们先前的研究表明，小鼠成纤维细胞暴露于原花青素(PA)1分钟即可促进细胞增殖，且呈浓度依赖关系。在这项研究中，人牙龈成纤维细胞(HGFs)暴露在PA中1分钟，同样地加速了细胞的增殖反应。除了加速增殖反应外，PA对暴露在恶劣环境条件下的HGFs具有细胞保护作用；短期将处于有丝分裂期的HGFs暴露在纯水或生理盐水中会导致活细胞回收率较低。PA的预处理和伴随处理改善了暴露在纯水或生理盐水中的细胞的低恢复率。此外，加入PA 1min后，即使在无血清培养条件下，HGFs仍能正常增殖。在100%融合的HGFs中，在无血清培养条件下，细胞内的ROS水平较高，但PA预培养可抑制细胞内ROS的增加。因此，结果表明，短期PA治疗通过改善细胞内氧化应激反应，对暴露于恶劣环境条件下的HGFs起到细胞保护作用。
Our previous study showed that exposing mouse fibroblasts to proanthocyanidin (PA) for only 1 min accelerated cell proliferation in a concentration-dependent manner. In this study, exposing human gingival fibroblasts (HGFs) to PA for 1 min similarly accelerated the proliferative response of the cells. Besides the accelerated proliferative response, PA showed a cytoprotective effect on HGFs exposed to harsh environmental conditions; short-term exposure of HGFs in the mitotic phase to pure water or physiological saline resulted in a lower recovery of viable cells. Pretreatment and concomitant treatment with PA improved the low recovery of cells exposed to pure water or physiological saline. In addition, HGFs exposed to PA for 1 min proliferated well even after being cultured in serum-free medium. In 100% confluent HGFs, being cultured in serum-free medium resulted in a high intracellular reactive oxygen species (ROS) level, but pretreatment with PA prevented the cells from increasing intracellular ROS. Thus, the results suggest that a short-term PA treatment exerts cytoprotective effects on HGFs exposed to harsh environmental conditions by improving the intracellular oxidative stress response.
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影响因子：
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