Affinity and selectivity of anchovy antibacterial peptide for Staphylococcus aureus cell membrane lipid and its application in whole milk
Affinity and selectivity of anchovy antibacterial peptide for Staphylococcus aureus cell membrane lipid and its application in whole milk
复制标题
鳀鱼抗菌肽对金黄色葡萄球菌细胞膜脂的亲和力和选择性及其在全脂奶中的应用
DOI：
10.1016/j.foodcont.2016.08.009
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发表时间：
2017-02
期刊：
影响因子：
6
通讯作者：
Wenting Tang
中科院分区：
文献类型：
--
作者：
Chuanfen Pu;Wenting Tang
An anchovy antibacterial peptide ACWWP1 (GLSRLFTALK) has been previously shown to kill bacterial cells via membrane damage. The affinity and selectivity of ACWWP1 for cell membrane lipids and their effects on the activity were investigated. Thermodynamic analysis indicated that hydrophobic and electrostatic effects contributed to the partitioning of ACWWP1 in the membrane lipids. Confocal Raman microspectroscopy, fluorescence anisotropy and calcein leakage analysis suggested that the peptide induced the greatest change in the order parameters of theStaphylococcus aureuscell membrane, which was related to the relaxation of the lateral stacking and an increase in the fluidity of the phospholipid bilayer. The positively charged ACWWP1 could change the surface charge state of the membrane. Besides, ACWWP1 exhibited efficient antimicrobial activity forS. aureusin whole milk. The results suggest that ACWWP1 exhibits favorable bacterial cell membrane lipid selectivity and can be employed as an efficient method for the treatment of food poisoning caused by certain bacteria.
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影响因子：
4.2
作者：
Varona, Salima;Martin, Angel;Jose Cocero, Maria
通讯作者：
Jose Cocero, Maria
影响因子：
2.7
作者：
通讯作者：
--
DOI：
10.1016/0005-2736(77)90078-5
发表时间：
1977-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GABER, BP;PETICOLAS, WL
通讯作者：
PETICOLAS, WL
固定化细菌膜脂质体层析从水煮凤尾鱼中筛选新型阳离子抗菌肽
影响因子：
6.1
作者：
Tang, Wenting;Zhang, Hui;Qian, Haifeng
通讯作者：
Qian, Haifeng
影响因子：
4.7
作者：
S. Bonora;M. Foggia;M. Iafisco
通讯作者：
S. Bonora;M. Foggia;M. Iafisco