Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responses.
Characterization of NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress responses.
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NAC转录因子NtNAC028作为叶片衰老和逆境反应调节因子的特性。
DOI：
10.3389/fpls.2022.941026
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
NAC 蛋白构成最大的转录因子家族之一，参与植物发育和胁迫反应的调节。我们之前对烟草的转录组分析表明，在叶子变黄期间，NtNAC028 的表达显着增加。在这项研究中，我们发现 NtNAC028 在高盐度、脱水和脱落酸 (ABA) 胁迫下迅速上调，表明该基因在非生物胁迫反应中发挥着至关重要的作用。通过 CRISPR-Cas9 产生的 NtNAC028 功能丧失烟草植物表现出叶片衰老延迟以及对干旱和盐胁迫的耐受性增强。同时，NtNAC028过表达导致拟南芥叶片早衰和对非生物胁迫过敏，表明NtNAC028作为自然叶片衰老的正调节剂和胁迫耐受性的负调节剂发挥作用。此外，NtNAC028过表达的拟南芥植物在高盐度下表现出较低的抗氧化酶活性、较高的活性氧（ROS）和H2O2积累，导致盐胁迫处理后氧化损伤更加严重。另一方面，烟草中的 NtNAC028 突变导致 ROS 清除和非生物胁迫相关基因的表达上调，抗氧化酶活性更高，并增强对非生物胁迫的耐受性，表明 NtNAC028 可能通过介导 ROS 清除能力，作为植物胁迫反应的重要调节剂。总的来说，我们的结果表明 NtNAC028 在叶片衰老和对多种非生物胁迫的响应中发挥着关键的调节作用。
NAC proteins constitute one of the largest transcription factor families and are involved in regulation of plant development and stress responses. Our previous transcriptome analyses of tobacco revealed a significant increase in the expression of NtNAC028 during leaf yellowing. In this study, we found that NtNAC028 was rapidly upregulated in response to high salinity, dehydration, and abscisic acid (ABA) stresses, suggesting a vital role of this gene in abiotic stress response. NtNAC028 loss-of-function tobacco plants generated via CRISPR-Cas9 showed delayed leaf senescence and increased tolerance to drought and salt stresses. Meanwhile NtNAC028 overexpression led to precocious leaf senescence and hypersensitivity to abiotic stresses in Arabidopsis, indicating that NtNAC028 functions as a positive regulator of natural leaf senescence and a negative regulator of stress tolerance. Furthermore, NtNAC028-overexpressing Arabidopsis plants showed lower antioxidant enzyme activities, higher reactive oxygen species (ROS), and H2O2 accumulation under high salinity, resulted in more severe oxidative damage after salt stress treatments. On the other hand, NtNAC028 mutation in tobacco resulted in upregulated expression of ROS-scavenging and abiotic stress-related genes, higher antioxidant enzyme activities, and enhanced tolerance against abiotic stresses, suggesting that NtNAC028 might act as a vital regulator for plant stress response likely by mediating ROS scavenging ability. Collectively, our results indicated that the NtNAC028 plays a key regulatory role in leaf senescence and response to multiple abiotic stresses.
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