Prevention of influenza virus shedding and protection from lethal H1N1 challenge using a consensus 2009 H1N1 HA and NA adenovirus vector vaccine.
Prevention of influenza virus shedding and protection from lethal H1N1 challenge using a consensus 2009 H1N1 HA and NA adenovirus vector vaccine.
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DOI：
10.1016/j.vaccine.2011.07.073
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发表时间：
2011-09-16
期刊：
影响因子：
5.5
通讯作者：
Paessler, Slobodan
中科院分区：
文献类型：
--
作者：
Jones, Frank R.;Gabitzsch, Elizabeth S.;Xu, Younong;Balint, Joseph P.;Borisevich, Viktoriya;Smith, Jennifer;Smith, Jeanon;Peng, Bi-Hung;Walker, Aida;Salazar, Magda;Paessler, Slobodan
关键词：
针对2009年H1N1大流行病毒等新兴病原体的疫苗可以受益于当前的技术，如快速基因组测序，以构建最具生物相关性的疫苗。一种新的平台（Ad 5 [E1-，E2 b-]）已被用于诱导对各种抗原靶标的免疫应答。我们采用该载体平台表达来自2009年H1N1大流行病毒的血凝素（HA）和神经氨酸酶（NA）基因。这些序列是从病毒分离物序列设计的共有序列，并且疫苗被快速构建和生产。疫苗接种诱导小鼠产生H1N1免疫应答，从而提供对致命病毒攻击的保护。在雪貂中，接种疫苗可防止疾病发展，并显著降低鼻洗液中的病毒滴度。H1N1细胞介导的免疫以及抗体诱导与预防疾病症状和减少病毒复制相关。应评估Ad 5 [E1-，E2 b-]，以快速开发针对传染病的有效疫苗。
Vaccines against emerging pathogens such as the 2009 H1N1 pandemic virus can benefit from current technologies such as rapid genomic sequencing to construct the most biologically relevant vaccine. A novel platform (Ad5 [E1-, E2b-]) has been utilized to induce immune responses to various antigenic targets. We employed this vector platform to express hemagglutinin (HA) and neuraminidase (NA) genes from 2009 H1N1 pandemic viruses. Inserts were consensuses sequences designed from viral isolate sequences and the vaccine was rapidly constructed and produced. Vaccination induced H1N1 immune responses in mice, which afforded protection from lethal virus challenge. In ferrets, vaccination protected from disease development and significantly reduced viral titers in nasal washes. H1N1 cell mediated immunity as well as antibody induction correlated with the prevention of disease symptoms and reduction of virus replication. The Ad5 [E1-, E2b-] should be evaluated for the rapid development of effective vaccines against infectious diseases.
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