Amide Proton Transfer-Weighted Magnetic Resonance Imaging for Detecting Severity and Predicting Outcome after Traumatic Brain Injury in Rats.
Amide Proton Transfer-Weighted Magnetic Resonance Imaging for Detecting Severity and Predicting Outcome after Traumatic Brain Injury in Rats.
复制标题
DOI：
10.1089/neur.2021.0064
复制
发表时间：
2022
影响因子：
2.4
通讯作者：
Zhou, Jinyuan
中科院分区：
文献类型：
--
作者：
Dong, Yinfeng;Gu, Yanting;Lu, Jianhua;Wan, Jieru;Jiang, Shanshan;Koehler, Raymond C.;Wang, Jian;Zhou, Jinyuan
关键词：
创伤性脑损伤（TBI）后，早期评估继发性损伤的严重程度是至关重要的估计预后和治疗分层。目前，二次伤害的严重程度难以估计。本研究的目的是探讨非侵入性酰胺质子转移加权（APTw）磁共振成像（MRI）技术评估TBI损伤在不同脑区和预测长期神经行为结果的能力。对55只雄性和雌性大鼠进行三种不同撞击器深度之一的受控皮质撞击，以产生不同程度的TBI。在1小时、1天和3天在4.7特斯拉扫描仪上采集多参数MRI数据。免疫荧光染色用于检测激活的小胶质细胞在第3天，并进行神经行为测试，以评估28天后的长期结果。在1天损伤核心的APTw信号与感觉运动功能、蔗糖偏好试验（一种快感缺乏试验）和巴恩斯迷宫空间记忆功能的缺陷相关。创伤后损伤侧皮层APTw信号逐渐增强，第3天的APTw值与第3天的小胶质细胞密度、第28天的空间记忆减退和快感缺失相关。在同侧丘脑中也观察到APTw与活化的小胶质细胞之间的相关性，并且在其他同侧部位中其与记忆缺陷和抑郁症的相关性是明显的。这些结果表明，APTw成像可用于检测继发性损伤，并作为TBI长期预后的潜在预测因子。
After traumatic brain injury (TBI), early assessment of secondary injury severity is critically important for estimating prognosis and treatment stratification. Currently, secondary injury severity is difficult to estimate. The objective of this study was to investigate the capacity of non-invasive amide proton transfer-weighted (APTw) magnetic resonance imaging (MRI) techniques to assess TBI injury in different brain regions and predict long-term neurobehavior outcomes. Fifty-five male and female rats were subjected to a controlled cortical impact with one of three different impactor depths to produce different degrees of TBI. Multi-parameter MRI data were acquired on a 4.7-Tesla scanner at 1 h, 1 day, and 3 days. Immunofluorescence staining was used to detect activated microglia at 3 days, and neurobehavioral tests were performed to assess long-term outcomes after 28 days. The APTw signal in the injury core at 1 day correlated with deficits in sensorimotor function, the sucrose preference test (a test for anhedonia), and spatial memory function on the Barnes maze. The APTw signal in the perilesion ipsilateral cortex gradually increased after TBI, and the value at 3 days correlated with microglia density at 3 days and with spatial memory decline and anhedonia at 28 days. The correlation between APTw and activated microglia was also observed in the ipsilateral thalamus, and its correlation to memory deficit and depression was evident in other ipsilateral sites. These results suggest that APTw imaging can be used for detecting secondary injury and as a potential predictor of long-term outcomes from TBI.
登录
查看更多内容
DOI：
10.1158/1078-0432.ccr-18-1233
发表时间：
2019-01-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Jiang S;Eberhart CG;Lim M;Heo HY;Zhang Y;Blair L;Wen Z;Holdhoff M;Lin D;Huang P;Qin H;Quinones-Hinojosa A;Weingart JD;Barker PB;Pomper MG;Laterra J;van Zijl PCM;Blakeley JO;Zhou J
通讯作者：
Zhou J
化学交换饱和转移 MRI 黑质信号丢失作为成像生物标志物评估帕金森病的诊断和服务
影响因子：
4.3
作者：
Li C;Chen M;Zhao X;Wang R;Chen H;Su W;Li S;Lou B;Song G;Zhang S;Zhang J;Zhou J
通讯作者：
Zhou J
影响因子：
4.2
作者：
Fong AK;Allen MD;Waltzman D;Sarmiento K;Yeates KO;Suskauer S;Wintermark M;Lindberg DM;Tate DF;Wilde EA;Loewen JL
通讯作者：
Loewen JL
影响因子：
4.2
作者：
Stone JR;Avants BB;Tustison NJ;Wassermann EM;Gill J;Polejaeva E;Dell KC;Carr W;Yarnell AM;LoPresti ML;Walker P;O'Brien M;Domeisen N;Quick A;Modica CM;Hughes JD;Haran FJ;Goforth C;Ahlers ST
通讯作者：
Ahlers ST
影响因子：
3.3
作者：
Heo HY;Zhang Y;Burton TM;Jiang S;Zhao Y;van Zijl PCM;Leigh R;Zhou J
通讯作者：
Zhou J