Maternal high-fat-diet programs rat offspring liver fatty acid metabolism.
Maternal high-fat-diet programs rat offspring liver fatty acid metabolism.
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DOI：
10.1007/s11745-015-4018-8
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发表时间：
2015-06
期刊：
影响因子：
1.9
通讯作者：
Desai, Mina
中科院分区：
文献类型：
--
作者：
Seet, Emily L.;Yee, Jennifer K.;Jellyman, Juanita K.;Han, Guang;Ross, Michael G.;Desai, Mina
在子宫内暴露于高脂肪（HF）母体饮食的后代中，我们以前已经证明，尽管出生体重相似，但与对照组相比，6个月大的HF成年后代体重显著增加，肥胖程度更高，三酰甘油（TAG）水平也增加。我们假设母亲HF饮食通过肝脏中单不饱和脂肪酸合成的程序性增加，从而增加肝脏TAG合成的底物可用性，使后代肥胖。我们进一步假设，后代肝脏脂肪酸代谢的程序性变化与脂肪生成酶硬脂酰辅酶A去饱和酶-1（SCD-1）的肝脏表达增加有关。雌性大鼠在妊娠前和整个妊娠期和哺乳期维持富含单不饱和脂肪酸（MUFA）的HF饮食。出生后，新生儿由同一母兽喂养，所有后代均断奶以控制饮食。采用气相色谱/质谱法测定血浆和肝脏脂肪酸组成。计算棕榈油酸/棕榈酸和（异油酸+棕榈油酸）/棕榈酸的脂肪酸C16去饱和指数和油酸/硬脂酸的C18去饱和指数。在新生儿和成年后代中分析SCD-1的肝脏蛋白丰度。HF新生儿血浆和肝脏C16去饱和指数降低，但成年后代增加。肝SCD-1的表达在HF成年后代中增加。这些数据表明，母亲在怀孕和哺乳期HF饮食增加后代肝脏SCD-1蛋白丰度和改变肝脏C16去饱和酶途径。
In offspring exposed in utero to a maternal diet high in fat (HF), we have previously demonstrated that despite similar birth weights, HF adult offspring at 6 months of age had significantly higher body weights, greater adiposity, and increased triacylglycerol (TAG) levels as compared to controls. We hypothesized that a maternal HF diet predisposes to offspring adiposity via a programmed increase in the synthesis of monounsaturated fatty acids in the liver and hence increased substrate availability for liver TAG synthesis. We further hypothesized that programmed changes in offspring liver fatty acid metabolism are associated with increased liver expression of the lipogenic enzyme stearoyl-CoA desaturase-1 (SCD-1). Female rats were maintained on a HF diet rich in monounsaturated fatty acids (MUFA) prior to and throughout pregnancy and lactation. After birth, newborns were nursed by the same dam, and all offspring were weaned to control diet. Plasma and liver fatty acid compositions were determined using gas chromatography/mass spectrometry. Fatty acid C16 desaturation indices of palmitoleic/palmitic and (vaccenic + palmitoleic)/palmitic and the C18 desaturation index of oleic/stearic were calculated. Liver protein abundance of SCD-1 was analyzed in newborns and adult offspring. Plasma and liver C16 desaturation indices were decreased in HF newborns, but increased in the adult offspring. Liver SCD-1 expression was increased in the HF adult offspring. These data show that the maternal HF diet during pregnancy and lactation increases offspring liver SCD-1 protein abundance and alters the liver C16 desaturase pathway.
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影响因子：
4.5
作者：
Jeyakumar SM;Lopamudra P;Padmini S;Balakrishna N;Giridharan NV;Vajreswari A
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Vajreswari A
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作者：
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通讯作者：
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