Lactobacillus bulgaricus mutants decompose uremic toxins
Lactobacillus bulgaricus mutants decompose uremic toxins
复制标题
保加利亚乳杆菌突变体分解尿毒症毒素
DOI：
10.3109/0886022x.2014.890111
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发表时间：
2014-05
期刊：
影响因子：
3
通讯作者：
Jiang Ya-Fen
中科院分区：
文献类型：
--
作者：
Jiang Yun-Sheng;Bai Yun-Huan;Jiang Ya-Fen
摘要背景/目的：本研究旨在通过检测保加利亚乳杆菌分解尿毒症毒素的能力获得一株益生菌，为慢性肾功能衰竭的治疗提供新的肠道细菌。方法：用尿毒症患者血清培养保加利亚乳杆菌，用物理(紫外线)和化学(硫酸二乙酯)方法反复诱变。以肌酐分解率为观察指标，筛选出肌酐降解率最高的菌株。然后我们测试了它分解尿素、尿酸、血清磷、甲状旁腺激素和同型半胱氨酸的能力以及它的遗传稳定性。结果：经诱变处理后，筛选出DUC3-17。对肌酐、尿素氮、尿酸、磷、甲状旁腺激素和同型半胱氨酸的分解率分别为17.23%、36.02%、9.84%、15.73%、78.26%和12.69%。这种降解能力持续了五代以上。结论：保加利亚乳杆菌经定向诱导和复合诱变后，具有较强的分解尿毒症毒素的能力，具有稳定的遗传特性。
Abstract Background/Aims: We aim to obtain a probiotic strain from Lactobacillus bulgaricus by testing its capability to decompose uremic toxins to provide new intestinal bacteria for the treatment of chronic renal failure. Methods: Original L. bulgaricus was cultured with the serum of uremic patients and then mutated by physical (ultraviolet) and chemical (diethyl sulfate) methods repeatedly. Using creatinine decomposition rate as an observed index, we selected the best strains which decreased the most concentration of the creatinine. We then tested its ability to decompose urea, uric acid, serum phosphate, parathyroid hormone, and homocysteine and its genetic stability. Results: After inductive and mutagenic treatment, DUC3-17 was selected. Its decomposition rate of creatinine, urea nitrogen, uric acid, phosphorus, parathyroid hormone, and homocysteine were 17.23%, 36.02%, 9.84%, 15.73%, 78.26%, and 12.69%, respectively. The degrading capacity was sustained over five generations. Conclusions: After directional induction and compound mutation, L. bulgaricus has greater capacity to decompose uremic toxins, with a stable inheritance.
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影响因子：
4.2
作者：
Liabeuf S;Drüeke TB;Massy ZA
通讯作者：
Massy ZA
DOI：
10.3109/10731190903356420
发表时间：
2009-01
期刊：
Artificial Cells, Blood Substitutes, and Biotechnology
影响因子：
--
作者：
Suai Zhang;Dongxia Li;K. Tian;YueXia Bai;Hongkai Zhang;Cunjiang Song;M. Qiao;D. Kong;Yao-ting Yu
通讯作者：
Suai Zhang;Dongxia Li;K. Tian;YueXia Bai;Hongkai Zhang;Cunjiang Song;M. Qiao;D. Kong;Yao-ting Yu
影响因子：
3.4
作者：
A. Mandal;Koushika Das;Suchismita Roy;K. C. Mondal;D. Nandi
通讯作者：
A. Mandal;Koushika Das;Suchismita Roy;K. C. Mondal;D. Nandi
影响因子：
4.2
作者：
Ranganathan, N;Patel, BG;Friedman, EA
通讯作者：
Friedman, EA
影响因子：
2.3
作者：
Ranganathan, Natarajan;Friedman, Eli A.;Dheer, Rahul
通讯作者：
Dheer, Rahul