An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.
An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.
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DOI：
10.1039/c3lc51303c
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发表时间：
2014-03-21
期刊：
影响因子：
6.1
通讯作者：
Walt DR
中科院分区：
文献类型：
--
作者：
Nie S;Henley WH;Miller SE;Zhang H;Mayer KM;Dennis PJ;Oblath EA;Alarie JP;Wu Y;Oppenheim FG;Little FF;Uluer AZ;Wang P;Ramsey JM;Walt DR
在过去的十年中，唾液已经成为一种潜在的理想的诊断生物液体，用于无创检测。在本文中，我们提出了一个自动化的综合平台，该平台可由在该领域受过最低限度培训的人员使用，用于使用人类唾液作为样本样本诊断呼吸道疾病。在这个平台上，唾液样本被加载到一个一次性微流控芯片上，该芯片包含唾液分析所需的所有必要试剂和成分。然后将芯片插入自动分析仪SDReader中，其中使用基于微球的阵列通过荧光夹心免疫测定同时测量多种潜在的呼吸系统疾病蛋白质生物标志物。结果是光学读取，并通过定制设计的算法分析图像。全自动分析只需要10 μL的唾液样本，结果在70分钟内报告。该平台的性能是通过测试蛋白质标准溶液来表征的，结果与我们之前报道的3.5小时实验室实验的结果相当。该设备还被部署在两个临床环境中，成功地测试了从不同受试者收集的273份人类唾液样本，证明了该设备通过提供及时的蛋白质生物标志物分析信息来帮助临床医生诊断呼吸道疾病的潜力。该平台结合了非侵入性样本采集和全自动分析，也可用于即时诊断。
During the last decade, saliva has emerged as a potentially ideal diagnostic biofluid for noninvasive testing. In this paper, we present an automated, integrated platform useable by minimally trained personnel in the field for the diagnosis of respiratory diseases using human saliva as a sample specimen. In this platform, a saliva sample is loaded onto a disposable microfluidic chip containing all the necessary reagents and components required for saliva analysis. The chip is then inserted into the automated analyzer, the SDReader, where multiple potential protein biomarkers for respiratory diseases are measured simultaneously using a microsphere-based array via fluorescence sandwich immunoassays. The results are read optically, and the images are analyzed by a custom-designed algorithm. The fully automated assay requires as little as 10 μL of saliva sample, and the results are reported in 70 min. The performance of the platform was characterized by testing protein standard solutions, and the results were comparable to those from the 3.5-h lab bench assay that we have previously reported. The device was also deployed in two clinical environments where 273 human saliva samples collected from different subjects were successfully tested, demonstrating the device’s potential to assist clinicians with the diagnosis of respiratory diseases by providing timely protein biomarker profiling information. This platform, which combines non-invasive sample collection and fully automated analysis, can also be utilized in point-of-care diagnostics.
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影响因子：
8.7
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