Integration of vascular progenitors into functional blood vessels represents a distinct mechanism of vascular growth.
Integration of vascular progenitors into functional blood vessels represents a distinct mechanism of vascular growth.
复制标题
血管祖细胞整合到功能性血管中代表了血管生长的独特机制。
DOI：
10.1016/j.devcel.2022.02.015
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发表时间：
2022-03-28
影响因子：
11.8
通讯作者：
Sumanas, Saulius
中科院分区：
文献类型：
--
作者：
Metikala, Sanjeeva;Warkala, Michael;Chetty, Satish Casie;Chestnut, Brendan;Florat, Diandra Rufin;Plender, Elizabeth;Nester, Olivia;Koenig, Andrew L.;Astrof, Sophie;Sumanas, Saulius
在胚胎发育过程中，最初的血管网络是通过血管发生的过程形成的，或者说是血管前体细胞的重新定位。相反，大多数后来形成的血管是由已经建立的血管系统形成的。在这里，我们展示了斑马鱼胚胎中新的血管前体细胞沿着卵黄延伸(称为次级血管场，SVF)出现，合并到主干后静脉，即使在血液循环开始后，也有助于肠道下血管的形成。我们进一步证明，SVF细胞参与了血管内皮细胞化学消融后的血管修复。血管祖细胞标志物ETV2/etsrp功能的诱导抑制阻止了SVF细胞的分化，导致肠下血管形成缺陷。在小鼠胚胎中也观察到了类似的晚期形成ETV2+祖细胞，这表明SVF细胞在进化上是保守的。我们的结果描述了新的血管前体细胞如何整合到已建立的血管系统中的一种不同的机制。梅蒂卡拉等人描述了新的晚期形成的血管前体细胞，这些细胞结合到斑马鱼胚胎中已建立的血管中。当所有其他内皮细胞都被切除时，这些细胞可以部分再生胚胎血管。作者鉴定了它们的转录图谱，并为哺乳动物胚胎中存在类似细胞提供了证据。
During embryogenesis, the initial vascular network forms by the process of vasculogenesis, or the specification of vascular progenitors de novo. In contrast, the majority of later-forming vessels arise by angiogenesis from the already established vasculature. Here we show that new vascular progenitors in zebrafish embryos emerge from a distinct site along the yolk extension (termed secondary vascular field, SVF), incorporate into the posterior cardinal vein and contribute to subintestinal vasculature even after blood circulation has been initiated. We further demonstrate that SVF cells participate in vascular recovery after chemical ablation of vascular endothelial cells. Inducible inhibition of vascular progenitor marker etv2 / etsrp function prevented SVF cell differentiation and resulted in the defective formation of subintestinal vasculature. Similar late-forming etv2+ progenitors were also observed in mouse embryos, suggesting that SVF cells are evolutionarily conserved. Our results characterize a distinct mechanism of how new vascular progenitors incorporate into established vasculature. Metikala et al describe new late-forming vascular progenitor cells which incorporate into established blood vessels in zebrafish embryos. These cells can partially regenerate embryonic vasculature when all other endothelial cells are ablated. The authors identify their transcriptional profile and provide evidence for the presence of similar cells in mammalian embryos.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
胚胎血管发育中的 ETS 转录因子。
影响因子：
9.8
作者：
Craig MP;Sumanas S
通讯作者：
Sumanas S
影响因子：
7
作者：
Auer TO;Duroure K;De Cian A;Concordet JP;Del Bene F
通讯作者：
Del Bene F
影响因子：
2.7
作者：
Dooley, KA;Davidson, AJ;Zon, LI
通讯作者：
Zon, LI
影响因子：
2.7
作者：
Davison, Jon M.;Akitake, Courtney M.;Parsons, Michael J.
通讯作者：
Parsons, Michael J.