SARS-CoV-2 in Exhaled Aerosol Particles from COVID-19 Cases and Its Association to Household Transmission.
SARS-CoV-2 in Exhaled Aerosol Particles from COVID-19 Cases and Its Association to Household Transmission.
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DOI：
10.1093/cid/ciac202
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发表时间：
2022-08-24
期刊：
影响因子：
--
通讯作者：
Löndahl J
中科院分区：
文献类型：
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作者：
Alsved M;Nygren D;Thuresson S;Medstrand P;Fraenkel CJ;Löndahl J
通过呼出气溶胶颗粒传播的2019冠状病毒病（COVID-19）被认为是感染传播的重要途径，特别是在涉及大声说话或唱歌的超级传播事件期间。然而，以前没有研究将病毒气溶胶排放的测量与传播率联系起来。2021年2月至3月期间，通过移动的实验室对接近症状发作的COVID-19病例进行了访问，分别收集呼吸、说话和唱歌时呼出的气溶胶颗粒以及鼻咽和唾液样本。使用BioSpot-VIVAS和NIOSH bc-251 2级旋风分离器收集气溶胶样品，并通过RT-qPCR分析所有样品用于严重急性呼吸综合征冠状病毒2（SARS-CoV-2）RNA检测。我们比较了气溶胶阳性和气溶胶阴性指数病例的家庭之间的传播率。38例病例中有19例（50%）在至少1份气溶胶样本中检测到SARS-CoV-2 RNA。发现阳性气溶胶样本的比值比（OR）从症状发作开始每天下降（OR 0.55，95置信区间[CI] .30-1.0，P =.049）。 阳性气溶胶样本数量最多的是唱歌，16（42%），其次是说话，11（30%），最少的是呼吸，3（8%）。在13个家庭的31名接触者中确定了指数病例。在有气溶胶阳性指数病例的家庭中观察到更高的传播率，10/16例感染（63%），而在有气溶胶阴性指数病例的家庭中，4/15例感染（27%）（χ2检验，P = 0.045）。 与呼吸相比，COVID-19病例更有可能在接近症状发作时以及在唱歌或说话时呼出含有SARS-CoV-2的气溶胶颗粒。这项研究支持呼出气溶胶中携带SARS-CoV-2的个体更有可能传播COVID-19。从空气样本缺失的病例报告中发现，Covid-19通过呼出的气溶胶颗粒传播。我们的研究量化了呼出空气中的SARS-CoV-2 RNA，并提供了阳性空气样本与家庭传播增加之间的直接联系。
Coronavirus disease 2019 (COVID-19) transmission via exhaled aerosol particles has been considered an important route for the spread of infection, especially during super-spreading events involving loud talking or singing. However, no study has previously linked measurements of viral aerosol emissions to transmission rates. During February–March 2021, COVID-19 cases that were close to symptom onset were visited with a mobile laboratory for collection of exhaled aerosol particles during breathing, talking, and singing, respectively, and of nasopharyngeal and saliva samples. Aerosol samples were collected using a BioSpot-VIVAS and a NIOSH bc-251 2-stage cyclone, and all samples were analyzed by RT-qPCR for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA detection. We compared transmission rates between households with aerosol-positive and aerosol-negative index cases. SARS-CoV-2 RNA was detected in at least 1 aerosol sample from 19 of 38 (50%) included cases. The odds ratio (OR) of finding positive aerosol samples decreased with each day from symptom onset (OR 0.55, 95 confidence interval [CI] .30–1.0, P = .049). The highest number of positive aerosol samples were from singing, 16 (42%), followed by talking, 11 (30%), and the least from breathing, 3 (8%). Index cases were identified for 13 households with 31 exposed contacts. Higher transmission rates were observed in households with aerosol-positive index cases, 10/16 infected (63%), compared to households with aerosol-negative index cases, 4/15 infected (27%) (χ2 test, P = .045). COVID-19 cases were more likely to exhale SARS-CoV-2-containing aerosol particles close to symptom onset and during singing or talking as compared to breathing. This study supports that individuals with SARS-CoV-2 in exhaled aerosols are more likely to transmit COVID-19. Transmission of covid-19 via exhaled aerosol particles were identified from case reports where air samples were missing. Our study quantifies SARS-CoV-2 RNA in exhaled air and provides a direct link between positive air samples and increased household transmission.
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DOI：
10.1093/cid/ciab797
发表时间：
2022-08-24
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Adenaiye OO;Lai J;Bueno de Mesquita PJ;Hong F;Youssefi S;German J;Tai SHS;Albert B;Schanz M;Weston S;Hang J;Fung C;Chung HK;Coleman KK;Sapoval N;Treangen T;Berry IM;Mullins K;Frieman M;Ma T;Milton DK
通讯作者：
Milton DK
影响因子：
4.9
作者：
Dumont-Leblond N;Veillette M;Bhérer L;Boissoneault K;Mubareka S;Yip L;Dubuis ME;Longtin Y;Jouvet P;McGeer A;Duchaine C
通讯作者：
Duchaine C
影响因子：
11.8
作者：
Katelaris AL;Wells J;Clark P;Norton S;Rockett R;Arnott A;Sintchenko V;Corbett S;Bag SK
通讯作者：
Bag SK
影响因子：
11.8
作者：
Park, Shin Young;Kim, Young-Man;Jeong, Eun Kyeong
通讯作者：
Jeong, Eun Kyeong
DOI：
10.3390/v13050908
发表时间：
2021-05-14
期刊：
Viruses
影响因子：
--
作者：
Basile L;Guadalupe-Fernández V;Valdivia Guijarro M;Martinez Mateo A;Ciruela Navas P;Mendioroz Peña J;The Epidemiological Surveillance Network Of Catalonia
通讯作者：
The Epidemiological Surveillance Network Of Catalonia