The Arabidopsis synaptotagmin SYTA regulates the cell-to-cell movement of diverse plant viruses.
The Arabidopsis synaptotagmin SYTA regulates the cell-to-cell movement of diverse plant viruses.
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DOI：
10.3389/fpls.2014.00584
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发表时间：
2014
影响因子：
5.6
通讯作者：
Lazarowitz SG
中科院分区：
文献类型：
--
作者：
Uchiyama A;Shimada-Beltran H;Levy A;Zheng JY;Javia PA;Lazarowitz SG
突触结合蛋白是动物中的一个大的基因家族，由于其作为钙传感器调节神经元中突触囊泡的胞吐和内吞作用，以及调节神经内分泌细胞的激素分泌的致密核心囊泡的胞吐作用，其已被广泛表征。突触结合蛋白被认为是动物所独有的，最近在拟南芥中被鉴定，其中它们包括一个五基因家族。使用感染性和基于叶的功能测定，我们已经表明拟南芥SYTA调节内吞作用并标记内体囊泡再循环途径，以通过胞间连丝调节Begomovirus卷心菜曲叶病毒（CaLCuV）和烟草花叶病毒（TMV）的运动蛋白介导的运输（刘易斯和Lazarowitz，）。为了确定SYTA是否在调节更广泛的不同植物病毒的细胞间运输中具有核心作用，我们在这里扩展了我们的研究，以检查SYTA在采用不同运动模式的其他植物病毒的细胞间运动中的作用，即马铃薯Y病毒属芜菁花叶病毒（TuMV），花椰菜花叶病毒花椰菜花叶病毒（CaMV）和烟草花叶病毒芜菁脉透明病毒（TVCV），与TMV相反，它们确实有效地感染拟南芥属。我们发现TuMV和TVCV的系统感染，以及它们的运动蛋白的细胞到细胞的运输，在拟南芥Col-0 syta-1敲除突变体中被延迟。相反，在syta-1中，CaMV系统性感染没有受到抑制。我们的研究表明，SYTA是植物病毒细胞间运动的关键调节因子，对于由菜豆病毒（CaLCuV MP）、烟草花叶病毒（TVCV和TMV 30 K蛋白）和马铃薯Y病毒（TuMV P3 N-PIPO）编码的不同细胞间运动蛋白改变PD并由此介导病毒细胞间传播的能力是必需的。
Synaptotagmins are a large gene family in animals that have been extensively characterized due to their role as calcium sensors to regulate synaptic vesicle exocytosis and endocytosis in neurons, and dense core vesicle exocytosis for hormone secretion from neuroendocrine cells. Thought to be exclusive to animals, synaptotagmins have recently been characterized in Arabidopsis thaliana, in which they comprise a five gene family. Using infectivity and leaf-based functional assays, we have shown that Arabidopsis SYTA regulates endocytosis and marks an endosomal vesicle recycling pathway to regulate movement protein-mediated trafficking of the Begomovirus Cabbage leaf curl virus (CaLCuV) and the Tobamovirus Tobacco mosaic virus (TMV) through plasmodesmata (Lewis and Lazarowitz,). To determine whether SYTA has a central role in regulating the cell-to-cell trafficking of a wider range of diverse plant viruses, we extended our studies here to examine the role of SYTA in the cell-to-cell movement of additional plant viruses that employ different modes of movement, namely the Potyvirus Turnip mosaic virus (TuMV), the Caulimovirus Cauliflower mosaic virus (CaMV) and the Tobamovirus Turnip vein clearing virus (TVCV), which in contrast to TMV does efficiently infect Arabidopsis. We found that both TuMV and TVCV systemic infection, and the cell-to-cell trafficking of the their movement proteins, were delayed in the Arabidopsis Col-0 syta-1 knockdown mutant. In contrast, CaMV systemic infection was not inhibited in syta-1. Our studies show that SYTA is a key regulator of plant virus intercellular movement, being necessary for the ability of diverse cell-to-cell movement proteins encoded by Begomoviruses (CaLCuV MP), Tobamoviruses (TVCV and TMV 30K protein) and Potyviruses (TuMV P3N-PIPO) to alter PD and thereby mediate virus cell-to-cell spread.
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影响因子：
64.5
作者：
Giordano F;Saheki Y;Idevall-Hagren O;Colombo SF;Pirruccello M;Milosevic I;Gracheva EO;Bagriantsev SN;Borgese N;De Camilli P
通讯作者：
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影响因子：
4.4
作者：
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通讯作者：
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作者：
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Laliberté JF
影响因子：
4.4
作者：
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通讯作者：
Craxton, M
影响因子：
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作者：
Fujiki, Masaaki;Kawakami, Shigeki;Beachy, Roger N.
通讯作者：
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