Fucosidases from the human gut symbiont Ruminococcus gnavus.
Fucosidases from the human gut symbiont Ruminococcus gnavus.
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DOI：
10.1007/s00018-020-03514-x
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Juge N
中科院分区：
文献类型：
--
作者：
Wu H;Rebello O;Crost EH;Owen CD;Walpole S;Bennati-Granier C;Ndeh D;Monaco S;Hicks T;Colvile A;Urbanowicz PA;Walsh MA;Angulo J;Spencer DIR;Juge N
岩藻糖基化聚糖在胃肠道内的可用性和再分配有助于肠道细菌物种适应生态位。为了获得这种营养来源，肠道细菌编码α-L-岩藻糖苷酶（岩藻糖苷酶），其催化末端α-L-岩藻糖苷键的水解。我们确定了从人类肠道共生体Ruminococcus gavus岩藻糖苷酶的底物和连接特异性。序列相似性网络鉴定了R. gnavus ATCC 29149和E1菌株，其针对一系列确定的寡糖和糖缀合物进行了进一步的酶法验证。使用聚糖微阵列、质谱、等温滴定量热法、结晶学和饱和转移差NMR方法的组合，我们鉴定了一种岩藻糖苷酶，其具有识别唾液酸封端的岩藻糖基化聚糖（唾液酸刘易斯X/A表位）并水解这些底物中的α1-3/4岩藻糖基键而无需去除唾液酸的能力。分子动力学模拟和分子对接表明，3′-Sialyl刘易斯X（sLeX）可以容纳在酶的结合位点内.这种特异性可能有助于R. gnavus菌株的婴儿和成人肠道，并在糖尿病和某些癌症的诊断糖组学测定中具有潜在的应用。本文的在线版本（10.1007/s 00018 -020-03514-x）包含补充材料，可供授权用户使用。
The availability and repartition of fucosylated glycans within the gastrointestinal tract contributes to the adaptation of gut bacteria species to ecological niches. To access this source of nutrients, gut bacteria encode α-l-fucosidases (fucosidases) which catalyze the hydrolysis of terminal α-l-fucosidic linkages. We determined the substrate and linkage specificities of fucosidases from the human gut symbiont Ruminococcus gnavus. Sequence similarity network identified strain-specific fucosidases in R. gnavus ATCC 29149 and E1 strains that were further validated enzymatically against a range of defined oligosaccharides and glycoconjugates. Using a combination of glycan microarrays, mass spectrometry, isothermal titration calorimetry, crystallographic and saturation transfer difference NMR approaches, we identified a fucosidase with the capacity to recognize sialic acid-terminated fucosylated glycans (sialyl Lewis X/A epitopes) and hydrolyze α1–3/4 fucosyl linkages in these substrates without the need to remove sialic acid. Molecular dynamics simulation and docking showed that 3′-Sialyl Lewis X (sLeX) could be accommodated within the binding site of the enzyme. This specificity may contribute to the adaptation of R. gnavus strains to the infant and adult gut and has potential applications in diagnostic glycomic assays for diabetes and certain cancers. The online version of this article (10.1007/s00018-020-03514-x) contains supplementary material, which is available to authorized users.
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DOI：
10.1107/s0907444905036693
发表时间：
2006-01-01
影响因子：
2.2
作者：
Evans, P
通讯作者：
Evans, P
DOI：
10.1107/s2059798317003382
发表时间：
2017-03-01
期刊：
Acta crystallographica. Section D, Structural biology
影响因子：
--
作者：
Emsley P
通讯作者：
Emsley P
DOI：
10.1016/j.bbapap.2015.04.015
发表时间：
2015-08
影响因子：
3.2
作者：
Gerlt, John A.;Bouvier, Jason T.;Davidson, Daniel B.;Imker, Heidi J.;Sadkhin, Boris;Slater, David R.;Whalen, Katie L.
通讯作者：
Whalen, Katie L.
影响因子：
4.6
作者：
Doherty M;Theodoratou E;Walsh I;Adamczyk B;Stöckmann H;Agakov F;Timofeeva M;Trbojević-Akmačić I;Vučković F;Duffy F;McManus CA;Farrington SM;Dunlop MG;Perola M;Lauc G;Campbell H;Rudd PM
通讯作者：
Rudd PM
影响因子：
4.8
作者：
Hobbs, Joanne K.;Pluvinage, Benjamin;Boraston, Alisdair B.
通讯作者：
Boraston, Alisdair B.