Hantavirus Gn and Gc envelope glycoproteins: key structural units for virus cell entry and virus assembly.
Hantavirus Gn and Gc envelope glycoproteins: key structural units for virus cell entry and virus assembly.
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DOI：
10.3390/v6041801
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发表时间：
2014-04-21
期刊：
影响因子：
--
通讯作者：
Tischler ND
中科院分区：
文献类型：
--
作者：
Cifuentes-Muñoz N;Salazar-Quiroz N;Tischler ND
近年来，对布尼亚病毒科中三个不同属的病毒表面刺突的超微结构研究表明，它们的刺突组织具有显著的多样性。尽管存在这种结构异质性，但在每种情况下，突刺似乎都是由Gn和Gc包膜糖蛋白形成的异源二聚体组成的。本文综述了Gn和Gc的结构及其在病毒细胞进入和退出中的作用。在病毒进入细胞过程中，Gn和Gc在受体结合中的作用尚未确定。然而，生化研究表明，随后的病毒-膜融合活性是由Gc完成的。此外，预测了汉坦病毒Gc的II类融合蛋白构象，并且新的晶体学数据证实了裂谷热病毒（RVFV） Gc蛋白的这种折叠。在病毒细胞退出过程中，不同病毒成分的组装似乎是通过Gn和Gc细胞质尾部（CT）与病毒内部核糖核衣壳的相互作用建立的。此外，最近的研究结果表明，汉坦病毒糖蛋白自组装成病毒样颗粒，在病毒出芽过程中发挥了重要作用。总的来说，这些新的见解为更详细地了解汉坦病毒感染周期早期和后期阶段的Gn和Gc功能提供了重要信息。
In recent years, ultrastructural studies of viral surface spikes from three different genera within the Bunyaviridae family have revealed a remarkable diversity in their spike organization. Despite this structural heterogeneity, in every case the spikes seem to be composed of heterodimers formed by Gn and Gc envelope glycoproteins. In this review, current knowledge of the Gn and Gc structures and their functions in virus cell entry and exit is summarized. During virus cell entry, the role of Gn and Gc in receptor binding has not yet been determined. Nevertheless, biochemical studies suggest that the subsequent virus-membrane fusion activity is accomplished by Gc. Further, a class II fusion protein conformation has been predicted for Gc of hantaviruses, and novel crystallographic data confirmed such a fold for the Rift Valley fever virus (RVFV) Gc protein. During virus cell exit, the assembly of different viral components seems to be established by interaction of Gn and Gc cytoplasmic tails (CT) with internal viral ribonucleocapsids. Moreover, recent findings show that hantavirus glycoproteins accomplish important roles during virus budding since they self-assemble into virus-like particles. Collectively, these novel insights provide essential information for gaining a more detailed understanding of Gn and Gc functions in the early and late steps of the hantavirus infection cycle.
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影响因子：
4.8
作者：
Estrada, D. Fernando;Boudreaux, Daniel M.;De Guzman, Roberto N.
通讯作者：
De Guzman, Roberto N.
影响因子：
5
作者：
Cifuentes-Munoz, Nicolas;Darlix, Jean-Luc;Tischler, Nicole D.
通讯作者：
Tischler, Nicole D.
影响因子：
4.8
作者：
Carneiro, FA;Stauffer, F;Da Poian, AT
通讯作者：
Da Poian, AT
DOI：
10.1083/jcb.200806081
发表时间：
2008-10-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fritz R;Stiasny K;Heinz FX
通讯作者：
Heinz FX
DOI：
10.4269/ajtmh.1994.51.102
发表时间：
1994-07-01
影响因子：
3.3
作者：
ELLIOTT, LH;KSIAZEK, TG;PETERS, CJ
通讯作者：
PETERS, CJ