Sequence of the DNA-binding protein of a human subgroup E adenovirus (type 4): comparisons with subgroup A (type 12), subgroup B (type 7), and subgroup C (type 5).

Sequence of the DNA-binding protein of a human subgroup E adenovirus (type 4): comparisons with subgroup A (type 12), subgroup B (type 7), and subgroup C (type 5).
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人类 E 亚型腺病毒(4 型)的 DNA 结合蛋白序列:与 A 亚型(12 型)、B 亚型(7 型)和 C 亚型(5 型)的比较。

DOI:
10.1016/0042-6822(85)90055-8
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发表时间:
1985
期刊:
影响因子:
3.7
通讯作者:
Kitchingman,GR
Kitchingman,GR
中科院分区:
医学3区
文献类型:
--
作者:
Kitchingman,GR

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测定了4型腺病毒(Ad4)单链DNA结合蛋白基因的核苷酸序列。该基因编码512个氨基酸的蛋白质。将该氨基酸序列与先前确定的Ad5、AD12和Adz的氨基酸序列进行比较,可以鉴定出这四种血清型之间的保守区域。这些序列包括蛋白质羧基末端结构域中9、9和12个氨基酸的延伸;这些序列与原核生物的单链DNA结合蛋白中鉴定的序列相似,对蛋白质与单链DNA的相互作用至关重要。在氨基末端区域有一个由四个氨基酸组成的保守区,其序列与最近被认为与蛋白质核定位有关的SV40大T抗原的一个区域完全相同。其他可能对蛋白质的三维结构很重要的保守氨基酸也被鉴定出来。四种血清型的DBP在氨基末端区域的同源性为17.2%,在羧基末端区域的同源性为47.8%。四种血清型DBP的双向比较表明,Ad4的DBP与Adz的DBP关系最为密切。
The nucleotide sequence of the gene for the single-stranded DNA-binding protein of adenovirus type 4 (Ad4) has been determined. The gene codes for a protein of 512 amino acids. Comparison of the amino acid sequence with those previously determined for Ad5, Ad12, and Adz allowed identification of regions that are conserved between the four serotypes. These include stretches of 9, 9, and 12 amino acids in the carboxy-terminal domain of the protein; these sequences are similar to those identified in the single-stranded DNA-binding proteins of procaryotes as being important for interaction of the protein with single-stranded DNA. A conserved region of four amino acids in the amino-terminal domain is identical in sequence to a region of the SV40 large T antigen that has recently been implicated in the nuclear localization of the protein. Other conserved amino acids that may be important for the three-dimensional structure of the protein have also been identified. The overall homology between the DBPs of the four serotypes is 17.2% in the amino-terminal domain, 47.8% in the carboxy-terminal domain. Two-way comparisons between the DBPs of the four serotypes indicates that the DBP of Ad4 is most closely related to that of Adz.
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