Molecular characterization and gene expression patterns of retinoid receptors, in normal and regenerating tissues of the sea cucumber, Holothuria glaberrima.
Molecular characterization and gene expression patterns of retinoid receptors, in normal and regenerating tissues of the sea cucumber, Holothuria glaberrima.
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DOI：
10.1016/j.gene.2018.01.102
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发表时间：
2018-05-15
期刊：
影响因子：
3.5
通讯作者：
García-Arrarás JE
中科院分区：
文献类型：
--
作者：
Viera-Vera J;García-Arrarás JE
视黄酸受体（RAR）和类维生素A X受体（RXR）是配体介导的转录因子，在后生动物中同步复杂的信号网络。这两种核受体之间的二聚体形成介导共调节复合物的募集，协调发育和再生事件期间信号级联的进展。在本研究中，我们确定和特点的视黄酸的海参Holothuria glaberrima的受体，一个模型系统能够在成年期再生器官发生。分子特征表明，由于选择性剪接事件，存在三种RAR亚型和两种RXR亚型。各种分析包括：一级结构测序、系统发育分析、蛋白质结构域预测和多序列比对进一步证实了它们的同一性。本文鉴定的每种受体亚型的半定量逆转录PCR分析表明，类维生素A受体在所有取样的组织中表达：肠系膜、呼吸树、肌肉、性腺和消化道。在再生器官发生期间，两种受体（RAR-L和RXR-T）在后段中显示差异表达，而RAR-S在肠前段中差异表达。这项工作提出了第一个描述的组件中继视黄酸在这个模型系统中的信号。
Retinoic acid receptors (RAR) and retinoid X receptors (RXR) are ligand-mediated transcription factors that synchronize intricate signaling networks in metazoans. Dimer formation between these two nuclear receptors mediates the recruitment of co-regulatory complexes coordinating the progression of signaling cascades during developmental and regenerative events. In the present study we identified and characterized the receptors for retinoic acid in the sea cucumber Holothuria glaberrima; a model system capable of regenerative organogenesis during adulthood. Molecular characterizations revealed the presence of three isoforms of RAR and two of RXR as a consequence of alternative splicing events. Various analyses including: primary structure sequencing, phylogenetic analysis, protein domain prediction, and multiple sequence alignment further confirmed their identity. Semiquantitative reverse transcription PCR analysis of each receptor isoform herein identified showed that the retinoid receptors are expressed in all tissues sampled: the mesenteries, respiratory trees, muscles, gonads, and the digestive tract. During regenerative organogenesis two of the receptors (RAR-L and RXR-T) showed differential expression in the posterior segment while RAR-S is differentially expressed in the anterior segment of the intestine. This work presents the first description of the components relaying the signaling for retinoic acid within this model system.
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影响因子：
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DOI：
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