An improved nitrogen difference method for estimating biological nitrogen fixation in legume-based intercropping systems
An improved nitrogen difference method for estimating biological nitrogen fixation in legume-based intercropping systems
复制标题
一种改进的氮差法，用于估计豆类间作系统中的生物固氮作用
DOI：
10.1007/s00374-009-0418-3
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发表时间：
2010-03
影响因子：
6.5
通讯作者：
Li Long
中科院分区：
文献类型：
--
作者：
Zhang Fu-Suo;Li Chun-Jie;Yu Chang-Bing;Sun Jian-Hao;He X. H.;Li Yu-Ying;Li Long
The nitrogen difference method (NDM) for quantifying N2fixation, based on the same amount of soil N exploited by N2-fixing and non-N2-fixing plant, may not be suitable to plants with different root traits. We tested the reliability of NDM in legume-based intercropping systems by two field experiments in Northwest China. In experiment 1, faba bean (Vicia faba), pea (Pisum sativum), and soybean (Glycine max) grew solely or intercropped with maize (Zea mays) with two N application rates (0, 225 kg ha−1). The biomass of faba bean, pea, and maize was significantly increased, whereas that of soybean was decreased when intercropped than solely grown. Aggressivity analyses demonstrate greater N competition ability of faba bean and pea, but not soybean, than maize. An improved NDM (INDM) could mitigate these effects: $$ {N_{{\text{fix - int}}}} = \left[ {{N_{{\text{leg - int}}}} + \frac{{1 - x}}{x}{N_{{\text{ref - int}}}} - \frac{{{N_{{\text{ref - sole}}}}}}{x}} \right] + \left[ {{\text{soil}}{N_{{\text{leg - int}}}} + \frac{{1 - x}}{x}{\text{soil}}{N_{{\text{ref - int}}}} - \frac{{{\text{soil}}{N_{{\text{ref - sole}}}}}}{x}} \right] $$, wherexand 1 −xare planting area of legume and non-legume in the intercropping system. Compared to traditional NDM (TNDM, $$ {N_{{\text{fix - int}}}} = \left[ {{N_{{\text{leg - int}}}} - {N_{{\text{ref - sole}}}}} \right] + \left[ {{\text{soil}}{N_{{\text{leg - int}}}} - {\text{soil}}{N_{{\text{ref - sole}}}}} \right] $$), %Ndfa(N derived from air) by INDM was decreased by 54.3% and 39.8% for faba bean, 44.7% and 5.0% for pea, but increased by 113.5% and 191.0% for soybean at the two N application rates, indicating different %Ndfaquantifications between the two methods. In experiment 2, %Ndfaof sole or intercropped faba bean was quantified by TNDM, INDM, and15N natural abundance method (NA). The %Ndfaonly by INDM correlated significantly with that from NA. Both interspecific root interactions and N loss affect %Ndfaestimation. Our results suggested that INDM could be more suitable than TNDM for quantifying %Ndfaof a N2-fixing plant in intercropping systems.
The nitrogen difference method (NDM) for quantifying N2fixation, based on the same amount of soil N exploited by N2-fixing and non-N2-fixing plant, may not be suitable to plants with different root traits. We tested the reliability of NDM in legume-based intercropping systems by two field experiments in Northwest China. In experiment 1, faba bean (Vicia faba), pea (Pisum sativum), and soybean (Glycine max) grew solely or intercropped with maize (Zea mays) with two N application rates (0, 225 kg ha−1). The biomass of faba bean, pea, and maize was significantly increased, whereas that of soybean was decreased when intercropped than solely grown. Aggressivity analyses demonstrate greater N competition ability of faba bean and pea, but not soybean, than maize. An improved NDM (INDM) could mitigate these effects: $$ {N_{{\text{fix - int}}}} = \left[ {{N_{{\text{leg - int}}}} + \frac{{1 - x}}{x}{N_{{\text{ref - int}}}} - \frac{{{N_{{\text{ref - sole}}}}}}{x}} \right] + \left[ {{\text{soil}}{N_{{\text{leg - int}}}} + \frac{{1 - x}}{x}{\text{soil}}{N_{{\text{ref - int}}}} - \frac{{{\text{soil}}{N_{{\text{ref - sole}}}}}}{x}} \right] $$, wherexand 1 −xare planting area of legume and non-legume in the intercropping system. Compared to traditional NDM (TNDM, $$ {N_{{\text{fix - int}}}} = \left[ {{N_{{\text{leg - int}}}} - {N_{{\text{ref - sole}}}}} \right] + \left[ {{\text{soil}}{N_{{\text{leg - int}}}} - {\text{soil}}{N_{{\text{ref - sole}}}}} \right] $$), %Ndfa(N derived from air) by INDM was decreased by 54.3% and 39.8% for faba bean, 44.7% and 5.0% for pea, but increased by 113.5% and 191.0% for soybean at the two N application rates, indicating different %Ndfaquantifications between the two methods. In experiment 2, %Ndfaof sole or intercropped faba bean was quantified by TNDM, INDM, and15N natural abundance method (NA). The %Ndfaonly by INDM correlated significantly with that from NA. Both interspecific root interactions and N loss affect %Ndfaestimation. Our results suggested that INDM could be more suitable than TNDM for quantifying %Ndfaof a N2-fixing plant in intercropping systems.
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