Phosphite utilization by the globally important marine diazotroph Trichodesmium.
Phosphite utilization by the globally important marine diazotroph Trichodesmium.
复制标题
全球重要的海洋固氮菌毛藻对亚磷酸盐的利用。
DOI:
10.1111/1758-2229.12308
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发表时间:
2015
影响因子:
3.3
通讯作者:
Polyviou D
中科院分区:
文献类型:
--
作者:
Polyviou D
Species belonging to the filamentous cyanobacterial genusTrichodesmiumare responsible for a significant fraction of oceanic nitrogen fixation. The availability of phosphorus (P) likely constrains the growth ofTrichodesmiumin certain regions of the ocean. Moreover,Trichodesmiumspecies have recently been shown to play a role in an emerging oceanic phosphorus redox cycle, further highlighting the key role these microbes play in many biogeochemical processes in the contemporary ocean. Here, we show thatTrichodesmium erythraeumIMS101 can grow on the reduced inorganic compound phosphite as its sole source of P. The components responsible for phosphite utilization are identified through heterologous expression of theT. erythraeumIMS101Tery_0365–0368genes, encoding a putative adenosine triphosphate (ATP)‐binding cassette transporter and nicotinamide adenine dinucleotide (NAD)‐dependent dehydrogenase, in the model cyanobacteriaSynechocystissp. PCC6803. We demonstrate that only combined expression of both the transporter and the dehydrogenase enablesSynechocystisto utilize phosphite, confirming the function of Tery_0365‐0367 as a phosphite uptake system (PtxABC) and Tery_0368 as a phosphite dehydrogenase (PtxD). Our findings suggest that reported uptake of phosphite byTrichodesmiumconsortia in the field likely reflects an active biological process byTrichodesmium. These results highlight the diversity of phosphorus sources available toTrichodesmiumin a resource‐limited ocean.
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影响因子:
4.4
作者:
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通讯作者:
C. Lassen
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Lipman, DJ
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通讯作者:
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作者:
Moore, C. Mark;Mills, Matthew M.;Woodward, E. Malcolm S.
通讯作者:
Woodward, E. Malcolm S.