M148R and M149R are two virulence factors for myxoma virus pathogenesis in the European rabbit.
M148R and M149R are two virulence factors for myxoma virus pathogenesis in the European rabbit.
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M148R和M149R是欧洲兔子病毒发病机理的两个毒力因子。
DOI：
10.1051/vetres:2008049
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发表时间：
2009-01
影响因子：
4.4
通讯作者：
Camus-Bouclainville C
中科院分区：
文献类型：
--
作者：
Blanié S;Mortier J;Delverdier M;Bertagnoli S;Camus-Bouclainville C
粘液瘤病毒 (MYXV) 是痘病毒科的一员，是引起粘液瘤病的病原体，粘液瘤病是欧洲兔 (Oryctolagus cuniculus) 的一种致命疾病。 MYXV 具有线性双链 DNA 基因组，编码对逃避宿主免疫系统很重要的几个因子。其中，鉴定出四种锚蛋白（ANK）重复蛋白：M148R、M149R、M150R和M-T5。迄今为止，仅对 M150R 和 M-T5 作为关键毒力因子进行了研究和表征。本文首次介绍了 M148R 和 M149R 的特征。绿色荧光蛋白（GFP）融合使我们能够将它们定位在病毒环境中。有趣的是，M149R 仅存在于细胞质中，而 M148R 部分位于核仁中，这是 ANK 重复痘病毒蛋白的独特特征。为了评估它们在病毒致病性中的意义，在粘液瘤病毒的野生型 T1 株中构建了靶向 M148R、M149R 或两者缺失。兔和灵长类培养细胞以及原代兔细胞的体外感染使我们得出结论，M148R 和 M149R 不太可能与细胞向性或宿主范围功能有关。然而，体内实验表明它们是毒力因子，因为欧洲兔子感染突变病毒后，观察到临床症状出现延迟、存活时间增加和死亡率急剧下降。此外，组织学分析表明，M148R 在 MYXV 破坏宿主炎症反应中发挥作用。
Myxoma virus (MYXV), a member of the Poxviridae family, is the agent responsible for myxomatosis, a fatal disease in the European rabbit (Oryctolagus cuniculus). MYXV has a linear double-stranded DNA genome that encodes several factors important for evasion from the host immune system. Among them, four ankyrin (ANK) repeat proteins were identified: M148R, M149R, M150R and M-T5. To date, only M150R and M-T5 were studied and characterized as critical virulence factors. This article presents the first characterization of M148R and M149R. Green Fluorescent Protein (GFP) fusions allowed us to localize them in a viral context. Whereas M149R is only cytoplasmic, interestingly, M148R is in part located in the nucleolus, a unique feature for an ANK repeat poxviral protein. In order to evaluate their implication in viral pathogenicity, targeted M148R, M149R, or both deletions were constructed in the wild type T1 strain of myxoma virus. In vitro infection of rabbit and primate cultured cells as well as primary rabbit cells allowed us to conclude that M148R and M149R are not likely to be implicated in cell tropism or host range functions. However, in vivo experiments revealed that they are virulence factors since after infection of European rabbits with mutant viruses, a delay in the onset of clinical signs, an increase of survival time and a dramatic decrease in mortality rate were observed. Moreover, histological analysis suggests that M148R plays a role in the subversion of host inflammatory response by MYXV.
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影响因子：
1.6
作者：
Mercer, AA;Fleming, SB;Ueda, N
通讯作者：
Ueda, N
影响因子：
5
作者：
Bradley, RR;Terajima, M
通讯作者：
Terajima, M
DOI：
10.1002/prot.340170405
发表时间：
1993-12-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
BORK, P
通讯作者：
BORK, P
影响因子：
3.8
作者：
SZEKELY, L;JIANG, WQ;RINGERTZ, N
通讯作者：
RINGERTZ, N
影响因子：
5.4
作者：
Shisler, JL;Jin, XL
通讯作者：
Jin, XL