Diet-Microbiota Interactions Alter Mosquito Development.
Diet-Microbiota Interactions Alter Mosquito Development.
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DOI：
10.3389/fmicb.2021.650743
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发表时间：
2021
影响因子：
5.2
通讯作者：
Strand MR
中科院分区：
文献类型：
--
作者：
Martinson VG;Strand MR
肠道微生物和饮食都可以强烈影响多细胞动物的生物学，但由于许多动物所携带的复杂微生物群落和它们所消耗的营养可变的饮食，通常很难解开微生物-饮食相互作用。虽然理论和经验研究表明，更大的微生物群多样性对许多动物宿主有益，但在水生无脊椎动物中进行的测试很少。大多数蚊子物种在其幼年阶段是水生食虫动物，具有可变的微生物，并且消耗从营养丰富到营养贫乏的饮食。在这项研究中，我们制作了一个gnotobiotic模型，使我们能够研究特定肠道微生物和饮食之间的相互作用如何影响埃及伊蚊（黄热病蚊子）的健康。使用简化的七成员细菌群落（ALL 7）和各种实验室和天然蚊子饮食，我们允许幼虫蚊子在不同的微生物和饮食条件下发育，并测量其成年时间和成年大小。幼虫接种ALL 7或更复杂的社会发展类似时，喂养营养丰富的大鼠饲料或鱼食实验室饮食，而幼虫接种个别细菌成员的ALL 7社区表现出很少的差异，在发展时，喂养大鼠饲料饮食，但表现出很大的差异时，喂养鱼食饮食的性能。相比之下，ALL 7社区在很大程度上未能支持幼虫的生长喂养现场收集的碎屑饲料，除非补充额外的蛋白质或酵母。总的来说，我们的研究结果表明，蚊子的发育和健身强烈依赖于饮食和微生物群落组成。
Gut microbes and diet can both strongly affect the biology of multicellular animals, but it is often difficult to disentangle microbiota–diet interactions due to the complex microbial communities many animals harbor and the nutritionally variable diets they consume. While theoretical and empirical studies indicate that greater microbiota diversity is beneficial for many animal hosts, there have been few tests performed in aquatic invertebrates. Most mosquito species are aquatic detritivores during their juvenile stages that harbor variable microbiotas and consume diets that range from nutrient rich to nutrient poor. In this study, we produced a gnotobiotic model that allowed us to examine how interactions between specific gut microbes and diets affect the fitness of Aedes aegypti, the yellow fever mosquito. Using a simplified seven-member community of bacteria (ALL7) and various laboratory and natural mosquito diets, we allowed larval mosquitoes to develop under different microbial and dietary conditions and measured the resulting time to adulthood and adult size. Larvae inoculated with the ALL7 or a more complex community developed similarly when fed nutrient-rich rat chow or fish food laboratory diets, whereas larvae inoculated with individual bacterial members of the ALL7 community exhibited few differences in development when fed a rat chow diet but exhibited large differences in performance when fed a fish food diet. In contrast, the ALL7 community largely failed to support the growth of larvae fed field-collected detritus diets unless supplemented with additional protein or yeast. Collectively, our results indicate that mosquito development and fitness are strongly contingent on both diet and microbial community composition.
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影响因子：
15.5
作者：
Callens M;Watanabe H;Kato Y;Miura J;Decaestecker E
通讯作者：
Decaestecker E
影响因子：
5.2
作者：
Anderson TR;Pond DW;Mayor DJ
通讯作者：
Mayor DJ
影响因子：
4.6
作者：
Buck M;Nilsson LK;Brunius C;Dabiré RK;Hopkins R;Terenius O
通讯作者：
Terenius O
影响因子：
5.2
作者：
Chandler, James Angus;Liu, Rachel M.;Bennett, Shannon N.
通讯作者：
Bennett, Shannon N.
影响因子：
1.2
作者：
Blatch, Sydella A.;Meyer, Kyle W.;Harrison, Jon F.
通讯作者：
Harrison, Jon F.