Identification of early neuronal subpopulations in avian neural crest cell cultures.
Identification of early neuronal subpopulations in avian neural crest cell cultures.
复制标题
禽类神经嵴细胞培养物中早期神经元亚群的鉴定。
DOI：
10.1016/0012-1606(85)90455-5
复制
发表时间：
1985
影响因子：
2.7
通讯作者：
Weston,JA
中科院分区：
文献类型：
--
作者：
Girdlestone,J;Weston,JA
神经节苷脂被用作早期分化标记物，以研究脊椎动物中性嵴细胞群体中表型多样性是如何产生的。从神经嵴衍生物代谢标记的糖脂的色谱分析显示，神经胶质细胞前体合成由背根神经节神经元产生的神经节苷脂类型的特征子集。神经嵴培养物的神经节苷脂合成模式类似于神经胶质前体，但神经元的神经节苷脂特征也是可检测的。为了确定神经元神经节苷脂是否由神经嵴培养物中的每个细胞或由离散细胞亚群表达，用单克隆抗体A2 B5对嵴培养物进行免疫细胞化学染色，所述单克隆抗体A2 B5识别GQ组分中的神经元特异性神经节苷脂。发现A2 B5在培养1天后与从神经管外植体分离的迁移阶段神经嵴细胞的约1%结合。这些A2 B5+细胞是有丝分裂后的，表现出单极或双极神经元形态。在将来自1天嵴细胞簇的细胞再培养1-2天后，出现第二个更大（10-20%）的A2 B5+细胞群。这些细胞最初具有典型的小，星状的嵴细胞形态，但后来延伸出一个或多个突起。[3 H]胸苷掺入和细胞计数研究表明，这些细胞的前体在有丝分裂后并表达A2 B5免疫反应性之前，在培养中至少分裂一次。第二个A2 B5+人口没有出现在从2天大的神经管外植体分离的嵴细胞簇的二次培养。另一种识别神经节苷脂GD 3的单克隆抗体R24与感觉神经节培养物中的神经元和非神经元亚群结合。在神经嵴培养物中，R24结合到大的细胞亚群，但不结合到A2 B5+细胞。这种免疫染色模式的意义尚不清楚。亚群的早期出现，以及在各种条件下培养的神经嵴中存在的异质性表明，内在的细胞机制可能会在环境因素作用的神经嵴中产生亚群。
Gangliosides were employed as early differentiation markers to investigate how phenotypic diversity is generated in the vertebrate neutral crest cell population. Chromatographic analysis of metabolically labeled glycolipids from neural crest derivatives revealed that glia cell precursors synthesize a characteristic subset of the ganglioside types produced by dorsal root ganglion neurons. The ganglioside synthesis pattern of neural crest cultures is similar to that of glial precursors, but gangliosides characteristic of neurons are also detectable. To determine whether neuronal gangliosides are expressed by every cell in neural crest cultures, or by discrete cell subpopulations, crest cultures were stained immunocytochemically with monoclonal antibody A2B5 which recognizes a neuron-specific ganglioside in the GQ fraction. A2B5 was found to bind to about 1% of migratory stage neural crest cells isolated from neural tube explants after 1 day in culture. These A2B5+cells were postmitotic and exhibited a uni- or bipolar neuronal morphology. A second, larger (10–20%) population of A2B5+cells appears after culturing cells from 1-day crest cell clusters an additional 1–2 days. These cells initially have the typical small, stellate morphology of crest cells but later extend one or more processes. [3H]Thymidine incorporation and cell counting studies show that the precursors to these cells had divided at least once in culture before becoming postmitotic and expressing A2B5 immunoreactivity. The second A2B5+population does not appear in secondary cultures of crest cell clusters isolated from 2-day-old explants of neural tubes. Another monoclonal antibody, R24, which recognizes ganglioside GD3, binds to subpopulations of both neurons and nonneurons in sensory ganglion cultures. In neural crest cultures R24 binds to a large subpopulation of cells, but not to A2B5+ones. The significance of this immunostaining pattern is not yet understood. The early appearance of subpopulations, and the presence of heterogeneity in neural crest cultured under a variety of conditions suggest that intrinsic cellular mechanisms might generate subpopulations within the neural crest upon which environmental factors act.
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DOI：
--
发表时间：
1974
期刊：
影响因子：
--
作者：
R. Laine;K. Stellner;S. Hakomori
通讯作者：
S. Hakomori
早期鹌鹑神经嵴细胞之间的异质性。
影响因子：
2.9
作者：
Sieber-Blum,M;Sieber,F
通讯作者：
Sieber,F
凝集素与神经嵴细胞结合。
影响因子：
7.8
作者：
M. Sieber;A. M. Cohen
通讯作者：
A. M. Cohen
培养神经元中的神经节苷脂
影响因子：
3.3
作者：
Henri Dreyfus;J. Louis;S. Harth;Paul Mandel
通讯作者：
Paul Mandel
鹌鹑神经嵴细胞的克隆分析：它们是多能的，并且在没有非嵴细胞的情况下在体外分化。
影响因子：
2.7
作者：
M. Sieber;A. M. Cohen
通讯作者：
A. M. Cohen