Cell migration from the corticostriatal angle to the basal telencephalon in rat embryos
Cell migration from the corticostriatal angle to the basal telencephalon in rat embryos
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大鼠胚胎中细胞从皮质纹状体角迁移至基底端脑
DOI：
10.1097/00001756-200103260-00032
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发表时间：
2001
期刊：
影响因子：
1.7
通讯作者：
T. Kaneko
中科院分区：
文献类型：
--
作者：
N. Tamamaki;KouichiC . Nakamura;T. Kaneko
外侧皮质流（Lateral cortical stream，LCS）是哺乳动物胚胎端脑外侧皮质中的一种细胞迁移流。细胞迁移的目的地为腹外侧新皮质、梨状皮质、梨状核和屏状核。然而，与此同时，LCS的其他目的地也被认为位于端脑腹侧。因此，我们使用几种分子生物学技术的组合来研究LCS的其他目的地。使用修饰的绿色荧光蛋白（GFP）的表达载体引入到脑室区（VZ）周围的皮质纹状体角，切向和径向细胞迁移揭示在LCS。LCS内的放射状细胞迁移向腹外侧新皮质、梨状皮质和外侧嗅束水平提供细胞。在第二个实验中，我们将转染了Sema 3A表达载体的COS-1细胞注射到新皮层的一侧。向LCS目的地的细胞供应由于LCS中形成大的坏死而停止，这是由Sema 3A-COS细胞注射引发的，并且嗅结节中的致密细胞层在COS细胞注射侧收缩。这些数据表明，在嗅结节的致密细胞层中的大多数神经元通过LCS到达这一点。皮质纹状体角可能是LCS细胞的起源之一。
Lateral cortical stream (LCS) has been described as a flow of cell migration found in the lateral cortex of the embryonic telencephalon of mammals. The destinations of the cell migration were reported as the ventrolateral neocortex, the pyriform cortex, endopyriform nucleus and the claustrum. At the same time, however, other destinations of LCS have been suggested in the ventral telencephalon. Therefore, we investigated the additional destinations of the LCS using a combination of several molecular biological techniques. Using an expression vector of modified green fluorescent protein (GFP) introduced into the ventricular zone (VZ) around the corticostriatal angle, both tangential and radial cell migration was revealed in the LCS. The radial cell migration in the LCS supplied cells to the ventro-lateral neocortex, pyriform cortex and to the level of the lateral olfactory tract. In the second experiment, we injected COS-1 cells transfected with a Sema3A expression vector into one side of the neocortex. The cell supply to the destination of the LCS ceased due to the formation of a large necrosis in the LCS, which was triggered by the Sema3A–COS cell injection, and the dense cell layer in the olfactory tubercle shrunk on the side where COS cells were injected. These data indicated that the majority of neurons in the dense cell layer of the olfactory tubercle reached this point through the LCS. One of the origins of the cells in the LCS would be in the corticostriatal angle.
影响因子：
56.9
作者：
Anderson, SA;Eisenstat, DD;Rubenstein, JLR
通讯作者：
Rubenstein, JLR
影响因子：
56.9
作者：
WALSH, C;CEPKO, CL
通讯作者：
CEPKO, CL