Loss of Interleukin-6 Influences Transcriptional Immune Signatures and Alters Bacterial Colonization in the Skin.
Loss of Interleukin-6 Influences Transcriptional Immune Signatures and Alters Bacterial Colonization in the Skin.
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DOI：
10.3389/fmicb.2021.658980
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发表时间：
2021
影响因子：
5.2
通讯作者：
Gallucci RM
中科院分区：
文献类型：
--
作者：
Luckett-Chastain LR;King CJ;McShan WM;Gipson JR;Gillaspy AF;Gallucci RM
皮肤作为一个保护屏障，以抑制外来病原体的进入，同时托管各种微生物的环境。随着时间的推移，皮肤细胞，免疫细胞，细胞因子和微生物相互作用，以整合维持皮肤的物理和免疫屏障的过程。在本研究中，两个免疫学不同的小鼠品系C57 BL/6和BALB/c，以及一个品系的C57背景缺乏IL-6的基础表达的特点。此外，还评估了菌株之间的皮肤抗菌基因表达谱和皮肤细菌微生物组。对未处理的C57 BL/6（对照）、BALB/c和IL-6缺陷皮肤样品进行总RNA测序，发现菌株之间差异调节的基因超过3，400个。发现每种菌株调节其自身的与皮肤稳态相关的转录“谱”，并且还影响整体细菌定殖，如由每种菌株上存在的差异门所指示的。总之，这些数据不仅提供了不同小鼠品系的稳态皮肤中的转录变化的全面视图，而且还突出了品系差异的可能影响（例如，Th 1/Th 2平衡）以及IL-6在整体皮肤免疫和常驻微生物群体中的作用。
The skin functions as a protective barrier to inhibit the entry of foreign pathogens, all the while hosting a diverse milieu of microorganisms. Over time, skin cells, immune cells, cytokines, and microbes interact to integrate the processes of maintaining the skin’s physical and immune barrier. In the present study, the basal expression of two immunologically divergent mouse strains C57BL/6 and BALB/c, as well as a strain on the C57 background lacking IL-6, was characterized. Additionally, cutaneous antimicrobial gene expression profiles and skin bacterial microbiome were assessed between strains. Total RNA sequencing was performed on untreated C57BL/6 (control), BALB/c, and IL-6-deficient skin samples and found over 3,400 genes differentially modulated between strains. It was found that each strain modulated its own transcriptional “profile” associated with skin homeostasis and also influenced the overall bacterial colonization as indicated by the differential phyla present on each strain. Together, these data not only provide a comprehensive view of the transcriptional changes in homeostatic skin of different mouse strains but also highlight the possible influence of the strain differences (e.g., Th1/Th2 balance) as well as a role for IL-6 in overall skin immunity and resident microbial populations.
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影响因子：
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影响因子：
6.5
作者：
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影响因子：
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