Occlusal hypofunction causes periodontal atrophy and VEGF/VEGFR inhibition in tooth movement.
Occlusal hypofunction causes periodontal atrophy and VEGF/VEGFR inhibition in tooth movement.
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DOI：
10.2319/011712-45.1
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发表时间：
2013-01
期刊：
影响因子：
--
通讯作者：
Ono T
中科院分区：
文献类型：
--
作者：
Usumi-Fujita R;Hosomichi J;Ono N;Shibutani N;Kaneko S;Shimizu Y;Ono T
目的观察大鼠实验性牙齿移动过程中牙周膜微血管的变化及血管内皮生长因子A（VEGF-A）和血管内皮生长因子受体2（VEGFR-2）的表达。15周龄雄性SD大鼠分为正常咬合组和咬合功能减退组。在2周的咬合提升期后，在两组中使用10-gf钛镍合金闭合螺旋弹簧将大鼠第一磨牙近中移动。在牙齿移动后第0、1、2、3和7天，通过显微计算机断层扫描和免疫组织化学方法，使用CD 31、VEGF-A、VEGFR-2和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）方法，检查组织学变化。功能减退的磨牙倾斜比正常磨牙，并没有移动后的第1天的牙齿移动显着。正常牙牙周膜张力侧血管增多。在牙齿移动过程中，VEGF-A和VEGFR-2在正常牙齿中的免疫反应性大于功能减退牙齿。压缩力迅速引起牙周膜和血管内皮细胞凋亡的功能减退的牙齿，但在正常牙齿。咬合功能减退通过VEGF-A和VEGFR-2表达的减少诱导血管收缩，并且在牙齿移动期间发生PDL和血管细胞的凋亡。
To examine changes in microvasculature and the expression of vascular endothelial growth factor A (VEGF-A) and VEGF receptor 2 (VEGFR-2) in rat hypofunctional periodontal ligament (PDL) during experimental tooth movement. Twelve-week-old male Sprague-Dawley rats were divided into normal occlusion and occlusal hypofunction groups. After a 2-week bite-raising period, rat first molar was moved mesially using a 10-gf titanium-nickel alloy closed coil spring in both groups. On days 0, 1, 2, 3, and 7 after tooth movement, histologic changes were examined by micro–computed tomography and immunohistochemistry using CD31, VEGF-A, VEGFR-2, and the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) method. Hypofunctional molars inclined more than normal molars and did not move notably after day 1 of tooth movement. Blood vessels increased on the tension side of the PDL in normal teeth. Immunoreactivities for VEGF-A and VEGFR-2 in normal teeth were greater than those in hypofunctional teeth during tooth movement. Compressive force rapidly caused apoptosis of the PDL and vascular endothelial cells in hypofunctional teeth, but not in normal teeth. Occlusal hypofunction induces vascular constriction through a decrease in the expression of VEGF-A and VEGFR-2, and apoptosis of the PDL and vascular cells occurs during tooth movement.
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影响因子：
2.5
作者：
Góth, MI;Hubina, E;Tóth, BE
通讯作者：
Tóth, BE
影响因子：
2.5
作者：
Fuss, Z;Tsesis, I;Lin, S
通讯作者：
Lin, S
血管内皮生长因子可以替代巨噬细胞集落刺激因子来支持破骨细胞骨吸收。
影响因子：
15.3
作者：
Niida, S;Kaku, M;Amano, H;Yoshida, H;Kataoka, H;Nishikawa, S;Tanne, K;Maeda, N;Nishikawa, S;Kodama, H
通讯作者：
Kodama, H
影响因子：
3.5
作者：
Kaneko, S;Ohashi, K;Yanagishita, M
通讯作者：
Yanagishita, M
影响因子：
3.6
作者：
Lee, Joo-Hee;Um, Soyoun;Seo, Byoung Moo
通讯作者：
Seo, Byoung Moo