Asynchronous development of Zymoseptoria tritici infection in wheat.
Asynchronous development of Zymoseptoria tritici infection in wheat.
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DOI：
10.1016/j.fgb.2020.103504
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Steinberg G
中科院分区：
文献类型：
--
作者：
Fantozzi E;Kilaru S;Gurr SJ;Steinberg G
小麦发酵斑孢菌在感染过程中经历了 6 个形态学定义的阶段。位于表面的孢子和菌丝的存在时间长达 17/18 天。感染后 1 至 13 天通过气孔进入。叶肉质外体定殖在感染过程中不断增加。在给定时间，受感染的叶子中最多共存 5 个阶段。小麦发酵菌(Zymoseptoria tritici)会导致小麦发生小麦斑枯病。致病性始于孢子萌发，随后是菌丝侵入气孔、叶肉定植和子实体形成。此前发现，通过气孔进入植物在几天内以非同步方式发生，而后来的发育步骤，例如早期和晚期子实体的形成，据报道在时间上彼此跟随。这表明在孢子形成之前植物中的病原体种群是同步的。在这里，我们对感染期间的荧光小麦小麦 IPO323 衍生菌株进行了成像。我们描述了 6 个形态上不同的发育阶段，并确定了它们在受感染叶子中的丰度以及接种后的时间。这表明在任何给定时间，受感染组织中同时存在 3-5 个阶段。因此，病原体发育的后期阶段也在感染细胞群体中异步发生。在解释从受感染植物收集的转录组学或蛋白质组学数据时，这一点值得考虑。
Zymoseptoria tritici passes 6 morphologically defined stages during infection. Surface-located spores and hyphae are found for up to 17/18 days. Entry through stomata occurs from 1 to 13 days post infection. Mesophyll apoplast colonisation continuously increases during infection. Up to 5 stages co-exist in infected leaves at a given time. The fungus Zymoseptoria tritici causes Septoria tritici blotch of wheat. Pathogenicity begins with spore germination, followed by stomata invasion by hyphae, mesophyll colonization and fruiting body formation. It was previously found that entry into the plant via stomata occurs in a non-synchronized way over several days, while later developmental steps, such as early and late fruiting body formation, were reported to follow each other in time. This suggests synchronization of the pathogen population in planta prior to sporulation. Here, we image a fluorescent Z. tritici IPO323-derived strain during infection. We describe 6 morphologically distinct developmental stages, and determine their abundance in infected leaves, with time post inoculation. This demonstrates that 3-5 stages co-exist in infected tissues at any given time. Thus, later stages of pathogen development also occur asynchronously amongst the population of infecting cells. This merits consideration when interpreting transcriptomics or proteomics data gathered from infected plants.
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DOI：
10.1016/j.fgb.2015.04.002
发表时间：
2015-06
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
Steinberg G
通讯作者：
Steinberg G
DOI：
10.1016/j.fgb.2020.103487
发表时间：
2021-01
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
Fantozzi E;Kilaru S;Cannon S;Schuster M;Gurr SJ;Steinberg G
通讯作者：
Steinberg G
影响因子：
4.4
作者：
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通讯作者：
Palmisano G
影响因子：
3
作者：
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通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
Courbot, Mikael