Chloroplast-located BjFer1 together with anti-oxidative genes alleviate hydrogen peroxide and hydroxyl radical injury in cytoplasmic male-sterile Brassica juncea
Chloroplast-located BjFer1 together with anti-oxidative genes alleviate hydrogen peroxide and hydroxyl radical injury in cytoplasmic male-sterile Brassica juncea
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位于叶绿体的 BjFer1 与抗氧化基因一起减轻芥菜细胞质雄性不育中的过氧化氢和羟基自由基损伤
DOI：
10.1007/s11033-011-1200-9
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发表时间：
2012-04
影响因子：
2.8
通讯作者：
中科院分区：
文献类型：
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作者：
研究了芥菜型油菜细胞质雄性不育（CMS）中植物细胞如何调节氧化还原稳态。芥菜型油菜细胞质雄性不育系线粒体多个基因发生突变，表明细胞氧化还原稳态发生了变化。我们观察到，这是不相关的增加氧化应激所示的减少H（2）O（2）和（N）OH含量在这种类型的CMS。光和暗条件下，5日龄的水稻幼苗中，不育系的几个抗氧化基因的表达量均高于不育系。线粒体交替氧化酶途径没有被激活，这表明AOX1a基因在CMS中的表达没有增加。在光和暗条件下，Ferritin1基因的表达在5日龄的水稻幼苗中比在MF系中显著增强。因此，我们检测到30日龄叶片中的全铁含量CMS比MF系高。我们从芥菜型油菜中分离到了铁蛋白1（Ferritin1）基因，该基因定位于叶绿体，并由柠檬酸铁和H2O2诱导，而不是阿坝诱导。综上所述，我们认为BjFer1和几个抗氧化基因的表达增加可以保护细胞免受氧化胁迫。
We studied how plant cell modulated redox homeostasis in cytoplasmic male-sterility (CMS) Brassica juncea. The CMS Brassica juncea was identified to be mutated in several mitochondrial genes that suggested the changes of cell redox homeostasis. We observed that it was not associated with increased oxidative stress as shown by decreased H(2)O(2) and (∙)OH contents in this type of CMS. The expressions of several anti-oxidative genes were up-regulated in 5-day-old seedlings of CMS than MF lines under light and dark conditions. The mitochondrial alternative oxidase pathway was not activated, as indicated by no increased expression of AOX1a gene in CMS. Interestingly, the expression of Ferritin1 gene was markedly activated in 5-day-old seedlings of CMS than MF line under light and dark conditions. Consequently, we detected increased content of total iron in 30-day-old leaves in CMS than MF line. We isolated Ferritin1 orthologous gene from Brassica juncea, which was targeted to the chloroplast and induced by Fe-citrate and H(2)O(2), not ABA. Taken together, we proposed that increased expressions of BjFer1 and several antioxidant genes protected cell from oxidative stress in CMS Brassica juncea.
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DOI：
10.1007/978-1-4020-2400-9_14
发表时间：
2004
期刊：
--
影响因子：
--
作者：
L. Sweetlove;C. Foyer
通讯作者：
L. Sweetlove;C. Foyer
芥菜（Brassica juncea）的新orf220型细胞质雄性不育系中线粒体atpA基因发生改变
影响因子：
2.8
作者：
通讯作者：
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线粒体 nad2 基因与细胞质雄性不育芥菜 (Brassica juncea) 中 CMS 相关的 orfB 基因共转录
影响因子：
2.8
作者：
通讯作者：
--
影响因子：
1.7
作者：
M. Wydro;E. Kozubek;P. Lehmann
通讯作者：
M. Wydro;E. Kozubek;P. Lehmann
影响因子：
4.8
作者：
Gueguen, V;Macherel, D;Bourguignon, J
通讯作者：
Bourguignon, J