Mitochondrial atpA gene is altered in a new orf220-type cytoplasmic male-sterile line of stem mustard (Brassica juncea)
Mitochondrial atpA gene is altered in a new orf220-type cytoplasmic male-sterile line of stem mustard (Brassica juncea)
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芥菜（Brassica juncea）的新orf220型细胞质雄性不育系中线粒体atpA基因发生改变
DOI：
10.1007/s11033-007-9176-1
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发表时间：
2009-02
影响因子：
2.8
通讯作者：
中科院分区：
文献类型：
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作者：
本研究旨在通过对水稻茎杆细胞质雄性不育及其保持系的比较分析，确定可能与细胞质雄性不育相关的线粒体因素。茎用芥菜细胞质雄性不育系的花器官形态发生了显著的变异。线粒体atpA基因在不育系与保持系中发生了改变，其中保持系的atpA基因编码507个氨基酸。与其他物种线粒体atpA基因的同源性较高，与水稻（Oryza sativa）氨基酸组成的同源性高达94%。而不育系中只有429个氨基酸与保持系atpA基因同源性为83%。在其保持系中观察到两个拷贝，而在不育系中只观察到一个拷贝。线粒体atpA基因在不育系与保持系间的差异可能不是进化趋异的结果，而是线粒体重排的结果。北方杂交结果显示，线粒体atpA基因在CMS中表达下调。因此，线粒体ATP合成严重减少了一倍以上的cms茎芥菜表明在这种类型的cms线粒体ATP合成不足。因此，我们推测线粒体atpA基因的改变可能与该类型不育性有关。
The purpose of this research is to identify the probable mitochondrial factor associated with cytoplasmic male sterility (cms) by comparative analysis of cms and its isogenic maintainer lines in stem mustards. Dramatic variations in the morphology of floral organs were observed in cms stem mustard. Mitochondrial atpA gene was shown to be altered in cms compared with that in its maintainer line, of which mitochondrial atpA gene from its maintainer line was sequenced to encode 507 amino acids. It was indicative of high homology with mitochondrial atpA genes from other species, even as high as 94% in similarity with Oryza sativa in terms of amino acid constituents. However, only 429 amino acids were deduced in cms showing 83% similarity with atpA gene from its maintainer line. Two copies were observed in its maintainer line, but only one was found in cms. Such numerous differences of mitochondrial atpA gene between cms and its maintainer lines may not be the results of evolutionary divergence but the rearrangements of mitochondria. Expression of mitochondrial atpA gene was shown to be down-regulated in cms by using Northern blot. Consequently, mitochondrial ATP synthesis was severely decreased more than one fold in cms stem mustard indicating deficiency in mitochondrial ATP synthesis in this type of cms. Therefore, we deduced that mitochondrial atpA gene altered in cms could be associated with male-sterility in this type of cms.
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影响因子：
3.1
作者：
Dieterich, JH;Braun, HP;Schmitz, UK
通讯作者：
Schmitz, UK
影响因子：
5.4
作者：
T. Sakai;J. Imamura
通讯作者：
T. Sakai;J. Imamura
影响因子：
7.7
作者：
Sabar, M;Gagliardi, D;Leaver, CJ
通讯作者：
Leaver, CJ
影响因子：
5.1
作者：
Fujii, Sota;Komatsu, Setsuko;Toriyama, Kinya
通讯作者：
Toriyama, Kinya
DOI：
10.1515/bchm.1997.378.8.859
发表时间：
1997-08
期刊：
--
影响因子：
--
作者：
J. Marienfeld;M. Unseld;P. Brandt;A. Brennicke
通讯作者：
J. Marienfeld;M. Unseld;P. Brandt;A. Brennicke