Prognostic significance of fascin expression in advanced colorectal cancer: an immunohistochemical study of colorectal adenomas and adenocarcinomas.
Prognostic significance of fascin expression in advanced colorectal cancer: an immunohistochemical study of colorectal adenomas and adenocarcinomas.
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DOI：
10.1186/1471-2407-6-241
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发表时间：
2006-10-09
期刊：
影响因子：
3.8
通讯作者：
Adams JC
中科院分区：
文献类型：
--
作者：
Hashimoto Y;Skacel M;Lavery IC;Mukherjee AL;Casey G;Adams JC
肌成束蛋白是一种肌动蛋白成束蛋白，在细胞突起的形成以及间充质和神经元细胞的运动中发挥作用。肌成束蛋白在上皮细胞中通常含量较低或不存在，但在几种上皮肿瘤中表达上调，可能导致侵袭性表型。在这里，我们报告了肌成束蛋白表达与结直肠腺癌进展和通过 Ki67 指数测量的肿瘤细胞增殖相关的流行率和潜在临床意义。通过免疫组织化学分析 107 例结直肠腺瘤和 35 例腺癌的常规组织切片中肌成束蛋白和 Ki67 的表达。还使用组织微阵列研究了肌成束蛋白表达和 Ki67 增殖指数，该组织微阵列包含来自另外 158 个结直肠腺癌和 15 个腺瘤的核心，这些腺瘤与 CCF、IRB 批准的数据库相关，平均临床随访时间为 38 个月。通过Kaplan-Meier和Cox回归方法进行生存分析。正常结肠上皮不表达肌成束蛋白。在常规切片中，16% 的腺瘤和 26% 的腺癌在超过 10% 的肿瘤细胞中显示肌成束蛋白表达。在临床注释的肿瘤中，肌成束蛋白免疫反应性在位于近端结肠的肿瘤中更常见（p = 0.009），但与年龄、性别或 TNM 分期无关。肌成束蛋白免疫反应性强的 III/IV 期腺癌 (n = 62) 患者的预后比肌成束蛋白低或缺失的患者预后更差（肌成束蛋白阴性患者的 3 年总生存率为 11%，而肌成束蛋白阴性患者为 43%；p = 0.023）。在腺瘤中，肌成束蛋白和 Ki67 在细胞水平上往往呈负相关。这种趋势在腺癌中不太明显。肌成束蛋白在一定比例的腺瘤中表达上调，其表达通常是局部的。在晚期结直肠腺癌的子集中观察到强烈且弥漫的表达，这与 III 期和 IV 期患者的较短生存期相关。肌成束蛋白作为更具侵袭性的结直肠腺癌的早期生物标志物可能具有预后价值。
Fascin is an actin bundling protein with roles in the formation of cell protrusions and motility of mesenchymal and neuronal cells. Fascin is normally low or absent from epithelia, but is upregulated in several epithelial neoplasms where it may contribute to an invasive phenotype. Here, we report on the prevalence and potential clinical significance of fascin expression in relation to the progression of colorectal adenocarcinoma and to tumor cell proliferation as measured by Ki67 index. Conventional tissue sections of 107 colorectal adenomas and 35 adenocarcinomas were analyzed by immunohistochemistry for fascin and Ki67 expression. Fascin expression and Ki67 proliferation index were also investigated by use of a tissue microarray containing cores from a further 158 colorectal adenocarcinomas and 15 adenomas linked to a CCF, IRB-approved database with a mean of 38 months of clinical follow-up. Survival analysis was carried out by the Kaplan-Meier and Cox regression methods. Fascin was not expressed by the normal colonic epithelium. In conventional sections, 16% of adenomas and 26% of adenocarcinomas showed fascin expression in greater than 10% of the tumor cells. In the clinically-annotated tumors, fascin immunoreactivity was more common in tumors located in the proximal colon (p = 0.009), but was not associated with age, gender, or TNM stage. Patients with stage III/IV adenocarcinomas (n = 62) with strong fascin immunoreactivity had a worse prognosis than patients with low or absent fascin, (3-year overall survival of 11% versus 43% for fascin-negative patients; p = 0.023). In adenomas, fascin and Ki67 tended to be inversely correlated at the cellular level; this trend was less apparent in adenocarcinomas. Fascin is upregulated in a proportion of adenomas, where its expression is often focal. Strong and diffuse expression was seen in a subset of advanced colorectal adenocarcinomas that correlated with shorter survival in stage III and IV patients. Fascin may have prognostic value as an early biomarker for more aggressive colorectal adenocarcinomas.
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DOI：
10.1073/pnas.94.25.13927
发表时间：
1997-12-09
影响因子：
11.1
作者：
Merritt, AJ;Gould, KA;Dove, WF
通讯作者：
Dove, WF
影响因子：
11.5
作者：
Hashimoto, Y;Ito, T;Shimada, Y
通讯作者：
Shimada, Y
影响因子：
7.5
作者：
Maitra, A;Adsay, NV;Hruban, RH
通讯作者：
Hruban, RH
影响因子：
1.2
作者：
De Arcangelis, A;Georges-Labouesse, E;Adams, JC
通讯作者：
Adams, JC
影响因子：
56.9
作者：
Novelli, MR;Williamson, JA;Wright, NA
通讯作者：
Wright, NA