Identification of a universal antigen epitope of influenza A virus using peptide microarray.
Identification of a universal antigen epitope of influenza A virus using peptide microarray.
复制标题
使用肽微阵列鉴定甲型流感病毒的通用抗原表位
DOI：
10.1186/s12917-020-02725-5
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发表时间：
2021-01-07
影响因子：
2.6
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Wang Q;Sun Z;Li J;Qin T;Ma H;Chen S;Peng D;Liu X
血凝素是甲型流感病毒（IAV）的主要表面蛋白，HA 2在不同的IAV中相对保守。因此，基于HA 2蛋白的广谱表位鉴定具有重要意义。合成H5 N1亚型IAV A/Mallard/Huadong/S/2005株HA 2蛋白的重叠肽段，将其负载于修饰的硅胶膜上制成芯片，用不同亚型IAV的抗血清筛选通用表位。使用抗肽免疫血清和用氨基酸取代拯救的病毒通过蛋白质印迹进一步确认所选择的表位。结果表明，HA 2中H5第14肽的485-FYHKCDNECME-495与H1、H3、H4、H5、H6、H7、H8、H9和H10亚型IAV抗血清具有广谱结合活性。拯救病毒中氨基酸（K或D）的取代导致血清结合力降低，表明它们是血清结合活性的关键残基。在免疫表位数据库中，一些含有14-4肽的表位被证实为MHC-II限制性CD 4 T细胞表位，并具有释放IL-2或IFN的作用。该表位可作为一种新的通用检测靶点，并可作为疫苗设计的靶点，其产生免疫保护的能力有待进一步研究。在线版本包含补充材料，可通过10.1186/s12917-020-02725-5获得。
Hemagglutinin is a major surface protein in influenza A virus (IAV), and HA2 is relative conserved among different IAVs. It will be meaningful to identify broad-spectrum epitopes based on the HA2 protein. Overlapping peptides of the HA2 protein of the H5N1 IAV A/Mallard/Huadong/S/2005 were synthesized and loaded on modified silica gel film to form a microarray, and antisera against different subtypes of IAVs were used to screen universal epitopes. The selected epitope was further confirmed by western blotting using anti-peptide immune serum and viruses rescued with amino acid substitution. The results showed that 485-FYHKCDNECME-495 of the H5 14th peptide in HA2 had broad-spectrum binding activity with antisera against H1, H3, H4, H5, H6, H7, H8, H9, and H10 subtype IAV. Substitution of amino acids (K or D) in rescued viruses resulted in decreased serum binding, indicating that they were critical residues for serum binding activity. In Immune Epitope Database, some epitopes containing 14–4 peptide were confirmed as MHC-II-restricted CD4 T cell epitope and had effects on releasing IL-2 or IFN. The identified epitope should be a novel universal target for detection and vaccine design and its ability to generate immune protection needs further exploration. The online version contains supplementary material available at 10.1186/s12917-020-02725-5.
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影响因子：
5.8
作者：
Kumar A;Vn MA;Raut AA;Sood R;Mishra A
通讯作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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