Interleukins and IgA synthesis. Human and murine interleukin 6 induce high rate IgA secretion in IgA-committed B cells.
Interleukins and IgA synthesis. Human and murine interleukin 6 induce high rate IgA secretion in IgA-committed B cells.
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DOI：
10.1084/jem.169.6.2133
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发表时间：
1989-06-01
期刊：
影响因子：
--
通讯作者：
McGhee JR
中科院分区：
文献类型：
--
作者：
Beagley KW;Eldridge JH;Lee F;Kiyono H;Everson MP;Koopman WJ;Hirano T;Kishimoto T;McGhee JR
将新鲜分离的鼠PP B细胞与10种不同的细胞因子（包括IL-1 α、IL-2、IL-4、IL-5、IL-6、IL-7、IFN-γ、TNF-α和TGF-β）一起培养，以研究这些细胞因子在诱导IG合成中的可能作用。令人感兴趣的是，发现只有IL-5和小鼠重组（mr）和人重组（hr）IL-6增强伊加合成。mrIL-6或hrIL-6的作用大于mrIL-5。IL-6诱导循环mIgA+ PP B细胞分泌高水平的伊加（比对照增加约7倍）。重要的是发现mrIL-6对PP B细胞培养物分泌IgM或IgG的影响很小。hrIL-6也增加PP B细胞的伊加分泌，这种增强作用被山羊抗hrIL-6抗血清消除。mrIL-6不引起B细胞增殖，但诱导分泌伊加的B细胞数量急剧增加。同种型转换不是这种伊加合成显著增加的机制，因为mIgA-PP B细胞不被mrIL-6诱导分泌伊加。从这些研究中，我们得出结论，IL-6在促进PP B细胞向分泌IgA的浆细胞的终末分化中起重要作用。
Freshly isolated murine PP B cells were cultured with 10 different cytokines, including IL-1 alpha, IL-2, IL-4, IL-5, IL-6, IL-7, IFN- gamma, TNF-alpha, and TGF-beta, to investigate a possible role for these cytokines in induction of Ig synthesis. Of interest was the finding that only IL-5 and both mouse recombinant (mr) and human recombinant (hr) IL-6 enhanced IgA synthesis. The effect was greater with either mrIL-6 or hrIL-6 than with mrIL-5. IL-6 induced cycling mIgA+ PP B cells to secrete high levels of IgA (approximately 7-fold increase over control). Of importance was the finding that mrIL-6 had little effect on secretion of IgM or IgG by PP B cell cultures. hrIL-6 also increased IgA secretion by PP B cells and this enhancement was abolished by a goat anti-hrIL-6 antiserum. mrIL-6 did not cause B cell proliferation but induced a sharp increase in numbers of B cells secreting IgA. Isotype-switching was not a mechanism for this marked increase in IgA synthesis since mIgA- PP B cells were not induced to secrete IgA by mrIL-6. From these studies we conclude that IL-6 plays an important role in promoting the terminal differentiation of PP B cells to IgA-secreting plasma cells.
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影响因子：
2.2
作者：
CZERKINSKY, CC;NILSSON, LA;TARKOWSKI, A
通讯作者：
TARKOWSKI, A
影响因子：
64.8
作者：
HIRANO, T;YASUKAWA, K;KISHIMOTO, T
通讯作者：
KISHIMOTO, T
DOI：
10.1084/jem.140.4.966
发表时间：
1974-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Jones PP;Cebra JJ
通讯作者：
Cebra JJ
DOI：
10.1073/pnas.84.24.9035
发表时间：
1987-12-01
影响因子：
11.1
作者：
IKEBUCHI, K;WONG, GG;OGAWA, M
通讯作者：
OGAWA, M
影响因子：
64.8
作者：
KAWANO, M;HIRANO, T;KISHIMOTO, T
通讯作者：
KISHIMOTO, T