Transcriptomics-based identification and characterization of 11 CYP450 genes of Panax ginseng responsive to MeJA
Transcriptomics-based identification and characterization of 11 CYP450 genes of Panax ginseng responsive to MeJA
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基于转录组学的人参 11 个 CYP450 基因对 MeJA 响应的鉴定和表征
DOI：
10.1093/abbs/gmy120
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发表时间：
2018-10
影响因子：
3.7
通讯作者：
Luo Zhiyong
中科院分区：
文献类型：
--
作者：
Zeng Xu;Luo Tiao;Li Jijia;Li Gui;Zhou Donghua;Liu Tuo;Zou Xian;Aparna P;ey;Luo Zhiyong
细胞色素 P450 (CYP450s) 是单加氧酶的超家族，对于在植物中产生高度功能化的次生代谢产物至关重要，并有助于双子叶植物中专门的三萜生物合成的多样化。然而，由于CYP450超家族中存在大量成员，筛选和鉴定人参皂苷生物合成中确切的CYP450基因极具挑战性。因此，为了筛选参与人参皂苷生物合成的CYP450基因，我们在前期工作中利用二代测序技术创建了人参转录组数据集。在生物信息学分析的基础上，从数据集中筛选出16个推测的具有显着差异表达的CYP450基因并提交给GenBank，其中11个已被克隆。使用茉莉酸甲酯（MeJA）作为诱导子，通过定量实时逆转录聚合酶链反应（qRT-PCR）分析候选CYP450基因的表达谱。 qRT-PCR分析结果显示，一些CYP450基因的表达受到MeJA的强烈诱导，并且在不同处理时间点表现出不同的转录水平。同源性分析表明，每个假定的人参CYP450蛋白都有一个由E-E-R-F-P-R-G组成的保守结构域。本研究对CYP450基因进行筛选和克隆，丰富了CYP450基因资源，生物信息学分析结果为进一步鉴定CYP450参与人参皂苷生物合成的功能奠定了基础。此外，这项研究促进了微生物细胞工厂的建设，通过代谢工程提高人参皂苷的产量。
Cytochromes P450 (CYP450s), a superfamily of mono-oxygenases, are essential to generate highly functionalized secondary metabolites in plants and contribute to the diversification of specialized triterpenoid biosynthesis in eudicots. However, screening and identifying the exact CYP450 genes in ginsenoside biosynthesis is extremely challenging due to existence of large quantities of members in CYP450 superfamily. Therefore, to screen the CYP450 genes involved in ginsenoside biosynthesis, transcriptome dataset of Panax ginseng was created in our previous work using the technique of the next-generation sequencing. On the basis of bioinformatics analysis, 16 putative CYP450 genes with significant differential expression were screened from the dataset and submitted to GenBank, in which 11 of them have been cloned. Methyl jasmonate (MeJA) was used as an elicitor to analyze the expression profiles of candidate CYP450 genes by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The results of qRT-PCR analysis revealed that the expression of some CYP450 genes were strongly induced by MeJA and showed different transcription levels at different treatment time points. Homology analysis indicated that each putative CYP450 protein of P. ginseng has a conserved domain consisting of E-E-R-F-P-R-G. The CYP450 genes were screened and cloned here to enrich the resources of CYP450 genes, and the results of bioinformatics analysis provided a foundation to further identify the function of CYP450s involved in ginsenoside biosynthesis. Furthermore, this study facilitated the construction of microbial cell factories for increasing the production of ginsenosides by means of metabolic engineering.
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影响因子：
2.9
作者：
H. Moon;Kil-Soo Kim;Shin-Kyo Chung;Jong-Kuk Kim
通讯作者：
H. Moon;Kil-Soo Kim;Shin-Kyo Chung;Jong-Kuk Kim
影响因子：
5.6
作者：
Cao H;Nuruzzaman M;Xiu H;Huang J;Wu K;Chen X;Li J;Wang L;Jeong JH;Park SJ;Yang F;Luo J;Luo Z
通讯作者：
Luo Z
影响因子：
8.2
作者：
Shi, Jing;Xue, Wei;Li, Ke-xin
通讯作者：
Li, Ke-xin
影响因子：
13.8
作者：
Enfissi, EMA;Fraser, PD;Bramley, PM
通讯作者：
Bramley, PM
DOI：
10.1199/tab.0158
发表时间：
2012-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Ruiz-Sola, M Aguila;Rodriguez-Concepcion, Manuel
通讯作者：
Rodriguez-Concepcion, Manuel