Near-Infrared 1064 nm Laser Modulates Migratory Dendritic Cells To Augment the Immune Response to Intradermal Influenza Vaccine.
Near-Infrared 1064 nm Laser Modulates Migratory Dendritic Cells To Augment the Immune Response to Intradermal Influenza Vaccine.
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DOI：
10.4049/jimmunol.1601873
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发表时间：
2017-08-15
期刊：
影响因子：
--
通讯作者：
Kashiwagi S
中科院分区：
文献类型：
--
作者：
Morse K;Kimizuka Y;Chan MPK;Shibata M;Shimaoka Y;Takeuchi S;Forbes B;Nirschl C;Li B;Zeng Y;Bronson RT;Katagiri W;Shigeta A;Sîrbulescu RF;Chen H;Tan RYY;Tsukada K;Brauns T;Gelfand J;Sluder A;Locascio JJ;Poznansky MC;Anandasabapathy N;Kashiwagi S
近红外(NIR)激光对皮肤的短暂照射可以增强皮内接种的免疫反应，从而起到免疫佐剂的作用。尽管有证据表明，与传统佐剂一样，近红外激光佐剂具有激活皮肤中包括树突状细胞(DC)在内的天然亚群的能力，但其确切的免疫学作用机制在很大程度上尚不清楚。在这里，我们试图通过建立皮内接种流感疫苗的小鼠模型，并检查特定DC群体的遗传消融引起的反应的变化，来确定近红外激光佐剂的细胞靶点。我们发现，连续波(CW)近红外激光佐剂广泛调节迁移的DC群体，特别是增加和激活皮肤中的lang+和CD11b−lang−亚群，并且连续波近红外激光增强的抗体反应依赖于表达CCR2和langerin的DC亚群。相比之下，脉冲波(PW)近红外激光佐剂对迁移DC亚群的影响有限。我们的疫苗接种研究表明，连续波近红外激光的疗效明显优于脉冲激光，表明连续波近红外激光为移行DC提供了理想的免疫刺激微环境。这些结果证明了近红外激光佐剂根据其参数选择性地针对皮肤中特定的迁移性DC群体的独特能力，并强调了优化激光参数对近红外激光佐剂疫苗诱导的理想免疫保护的重要性。
Brief exposure of skin to near-infrared (NIR) laser light has been shown to augment the immune response to intradermal vaccination and thus act as an immunologic adjuvant. Although evidence indicates that the NIR laser adjuvant has capacity to activate innate subsets including dendritic cells (DCs) in skin as conventional adjuvants do, the precise immunological mechanism by which the NIR laser adjuvant acts is largely unknown. Here we sought to identify the cellular target of the NIR laser adjuvant by using an established mouse model of intradermal influenza vaccination and examining the alteration of responses resulting from genetic ablation of specific DC populations. We found that a continuous wave (CW) NIR laser adjuvant broadly modulates migratory DC populations, specifically increasing and activating the Lang+ and CD11b−Lang− subsets in skin, and that the antibody responses augmented by the CW NIR laser are dependent on DC subsets expressing CCR2 and Langerin. In comparison, a pulsed wave (PW) NIR laser adjuvant showed limited effects on the migratory DC subsets. Our vaccination study demonstrated that the efficacy of CW NIR laser is significantly better than that of PW laser, indicating that the CW NIR laser offers a desirable immunostimulatory microenvironment for migratory DCs. These results demonstrate the unique ability of the NIR laser adjuvant to selectively target specific migratory DC populations in skin depending on its parameters, and highlight the importance of optimization of laser parameters for desirable immune protection induced by a NIR laser-adjuvanted vaccine.
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DOI：
10.1084/jem.20131397
发表时间：
2014-08-25
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Anandasabapathy N;Feder R;Mollah S;Tse SW;Longhi MP;Mehandru S;Matos I;Cheong C;Ruane D;Brane L;Teixeira A;Dobrin J;Mizenina O;Park CG;Meredith M;Clausen BE;Nussenzweig MC;Steinman RM
通讯作者：
Steinman RM
影响因子：
3.7
作者：
Kashiwagi S;Yuan J;Forbes B;Hibert ML;Lee EL;Whicher L;Goudie C;Yang Y;Chen T;Edelblute B;Collette B;Edington L;Trussler J;Nezivar J;Leblanc P;Bronson R;Tsukada K;Suematsu M;Dover J;Brauns T;Gelfand J;Poznansky MC
通讯作者：
Poznansky MC
影响因子：
7.3
作者：
Awate S;Babiuk LA;Mutwiri G
通讯作者：
Mutwiri G
影响因子：
32.4
作者：
Jung, S;Unutmaz, D;Lang, RA
通讯作者：
Lang, RA
影响因子：
5.5
作者：
Kimizuka, Yoshifumi;Callahan, John J.;Kashiwagi, Satoshi
通讯作者：
Kashiwagi, Satoshi