Plasmodesmata targeting and intercellular trafficking of Tomato spotted wilt tospovirus movement protein NSm is independent of its function in HR induction.
Plasmodesmata targeting and intercellular trafficking of Tomato spotted wilt tospovirus movement protein NSm is independent of its function in HR induction.
复制标题
番茄斑萎病病毒运动蛋白 NSm 的胞间连丝靶向和细胞间运输与其在 HR 诱导中的功能无关。
DOI：
10.1099/jgv.0.000496
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发表时间：
2016-08
期刊：
影响因子：
--
通讯作者：
Xiaorong Tao
中科院分区：
文献类型：
--
作者：
Wenyang Zhao;Lei Jiang;Zhike Feng;Xiaojiao Chen;Ying Huang;Fan Xue;Changjun Huang;Yong Liu;Fan Li;Yating Liu;Xiaorong Tao
番茄斑点枯萎病病毒（TSWV）的运动蛋白NSm在病毒细胞间运输中起关键作用。最近，TSWV NSm在sw -5b介导的超敏反应（HR）中也被确定为毒性（Avr）决定因素。然而，NSm的细胞间运动是否与其在HR诱导中的功能相耦合仍不清楚。在这里，我们发现靶向胞间连丝和细胞间运动缺陷的NSm突变体仍然能够诱导sw -5b介导的HR。此外，将先前从TSWV耐药分离株中鉴定出的单氨基酸替换C118Y或T120N引入运动缺陷的NSm突变体中，导致HR诱导失败。总的来说，我们的研究结果表明，NSm的细胞间运输与其在HR诱导中的功能是分离的。这些发现揭示了R-Avr识别的进化机制，并可能用于解释为什么这种不耦合现象可以在许多不同的病毒中观察到。
The movement protein NSm of Tomato spotted wilt tospovirus (TSWV) plays pivotal roles in viral intercellular trafficking. Recently, the TSWV NSm was also identified as an avirulence (Avr) determinant during the Sw-5b-mediated hypersensitive response (HR). However, whether the cell-to-cell movement of NSm is coupled to its function in HR induction remains obscure. Here, we showed that the NSm mutants defective in targeting plasmodesmata and cell-to-cell movement were still capable of inducing Sw-5b-mediated HR. In addition, introduction of a single amino-acid substitution, C118Y or T120N, identified previously from TSWV resistance-breaking isolates, into the movement-defective NSm mutants resulted in the failure of HR induction. Collectively, our results showed that the intercellular trafficking of NSm is uncoupled from its function in HR induction. These findings shed light on the evolutionary mechanism of R-Avr recognition and may be used to explain why this uncoupled phenomenon can be observed in many different viruses.
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