"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.
"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.
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DOI：
10.1371/journal.pone.0072752
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Vaury C
中科院分区：
文献类型：
--
作者：
Dennis C;Zanni V;Brasset E;Eymery A;Zhang L;Mteirek R;Jensen S;Rong YS;Vaury C
皮尔纳途径通过沉默移动的元件来保护基因组。尽管在理解产生用于沉默的piRNA的加工事件方面取得了进展，但对初级转录物如何从其基因组簇转运到其加工中心知之甚少。使用果蝇COM/弗拉门戈基因座在卵巢体细胞的模型，我们确定了一个突出的核结构称为COM点，这是丰富的皮尔纳簇的长转录本，但位于远离其转录位点。值得注意的是，来自多个簇的转录物积累在Dot COM，其通常与簇转录物的细胞质处理中心Yb体并置。遗传证据表明，前体转录物在Dot COM的积累代表了皮尔纳途径中最上游的事件之一。我们的研究结果为皮尔纳通路的初始步骤提供了新的见解，并开辟了一个新的研究领域，对于完整理解这种保守的通路至关重要。
The piRNA pathway protects genomes by silencing mobile elements. Despite advances in understanding the processing events that generate piRNAs for silencing, little is known about how primary transcripts are transported from their genomic clusters to their processing centers. Using a model of the Drosophila COM/flamenco locus in ovarian somatic cells, we identified a prominent nuclear structure called Dot COM, which is enriched in long transcripts from piRNA clusters but located far from their transcription sites. Remarkably, transcripts from multiple clusters accumulate at Dot COM, which is often juxtaposed with Yb-bodies, the cytoplasmic processing centers for cluster transcripts. Genetic evidence suggests that the accumulation of precursor transcripts at Dot COM represents one of the most upstream events in the piRNA pathway. Our results provide new insights into the initial steps of the piRNA pathway, and open up a new research area important for a complete understanding of this conserved pathway.
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