Characterization of the oxygenated intermediate of the thermophilic cytochrome P450 CYP119.
Characterization of the oxygenated intermediate of the thermophilic cytochrome P450 CYP119.
复制标题
嗜热细胞色素 P450 CYP119 含氧中间体的表征。
DOI：
10.1016/s0162-0134(01)00328-2
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发表时间：
2001
影响因子：
3.9
通讯作者：
Sligar,SG
中科院分区：
文献类型：
--
作者：
Denisov,IG;Hung,SC;Weiss,KE;McLean,MA;Shiro,Y;Park,SY;Champion,PM;Sligar,SG
Using UV–Vis, resonance Raman, and EPR spectroscopy we have studied the properties of the oxygenated ferrous cytochrome P450 from Sulfolobus Solfataricus, (CYP119). The recently determined crystal structure of CYP119 is compared with other available structures of P450s, and detailed structural and spectroscopic analyses are reported. With several structural similarities to CYP102, such as in-plane iron position and a shorter iron-proximal ligand bond, CYP119 shows low-spin conformation preference in the ferric form and partially in the ferrous form at low temperatures. These structural features can explain the fast autoxidation of the oxyferrous complex of CYP119. Finally, we report the first UV–Vis and EPR spectra of the cryoradiolytically reduced oxygenated intermediate of CYP119. The primary reduced intermediate, a hydroperoxo–ferric complex of CYP119, undergoes a ‘peroxide shunt’ pathway during gradual annealing at 170–195 K and returns to the low-spin ferric form.
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影响因子：
2.9
作者：
CHAMPION, PM;LIPSCOMB, JD;GUNSALUS, IC
通讯作者：
GUNSALUS, IC
低温氧-血红素复合物单电子还原的中间自旋态
影响因子：
3.5
作者：
Zbigniew Gasyna
通讯作者：
Zbigniew Gasyna
影响因子：
3.3
作者：
Debije, MG;Bernhard, WA
通讯作者：
Bernhard, WA
物理化学方法调控细胞色素P450活性
DOI：
--
发表时间：
2000
期刊：
影响因子：
--
作者：
V. V. Shumyantseva;T. Bulko;A. Archakov
通讯作者：
A. Archakov
DOI：
10.1016/s0006-291x(77)80131-9
发表时间：
1977-01-01
影响因子：
3.1
作者：
EISENSTEIN, L;DEBEY, P;DOUZOU, P
通讯作者：
DOUZOU, P