The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration
The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration
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培养中的人胎儿嗅球和嗅鞘细胞作为临床中枢神经系统恢复来源的免疫组织化学特征
DOI：
10.1002/ar.21030
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Changman Zhou
中科院分区：
文献类型：
--
作者：
Kai Liu;Ying Li;Hongmei Wang;Xiaorong Jiang;Yanting Zhao;Dongming Sun;Lin Chen;W. Young;Hongyun Huang;Changman Zhou
临床研究已将嗅鞘细胞(OECs)移植扩大到不同的人类中枢神经系统(CNS)疾病。事实上，临床上的嗅鞘细胞移植是嗅球细胞的混合体，他们甚至还没有证明自己有这样的亚群。然而，作为嗅鞘细胞移植的来源，人胎儿嗅鞘细胞的培养和鉴定仍需要更多的了解，因为一些手术试图通过一些不同的程序产生更纯净的嗅鞘细胞培养物来修复中枢神经系统损伤。本文对6例妊娠20周的人胎儿嗅球(OB)标本进行了免疫荧光染色和免疫荧光染色，并在激光共聚焦显微镜下对培养的细胞进行了观察。P75NTR阳性的OB-OECs(来自嗅球的嗅鞘细胞)在外嗅神经层和肾小球层均有表达。培养的OB细胞中p75NTR阳性细胞占22.31%，S100β阳性细胞占45.77%，GFAP阳性细胞占31.92%。在人胚胎嗅鞘细胞中，p75NTR和GFAP分别与S100β共表达，而p75NTR和GFAP不共表达。提示人嗅鞘细胞在成骨细胞中的定位和发育与啮齿动物不同，p75NTR免疫组织化学染色仍有必要在移植前对培养中的人胎儿嗅鞘细胞进行鉴定和鉴定。Anat Rec，2010年。©2009威利-利斯，Inc.
Clinical studies have expanded the therapeutic olfactory ensheathing cells (OECs) transplantation to different human Central Nervous System (CNS) diseases. In fact, the OEC transplantation in clinic is a mixture of olfactory bulb cells; they even have not demonstrated that they have such a subpopulation yet. However, as a source of OECs transplantation, the development and identification of human fetal OECs are still need more understanding, because some surgery try to restoration CNS injury with a more purity of OEC cultures generated by a number of different procedures. In this article, twelve human fetal olfactory bulb (OB) samples were obtained from six fetuses in 20 weeks of gestation, it was studied by immunofluorescence on histological sections and cultured cells with multiple antibodies under confocal microscopy. The P75NTR positive OB‐OECs (olfactory ensheathing cell from the olfactory bulb) were present in both outer olfactory nerve layers and glomerular layer. The percentage of OB cells in culture, about 22.31 was P75NTR positive, 45.77 was S100β, and 31.92 was GFAP. P75NTR and GFAP were coexpressed with S100β, respectively; however, P75NTR was not coexpressed with GFAP in human fetal OECs. It is suggested that the localization and development of human OECs in OB are different to those in rodent, and the P75NTR immunohistological staining is still necessary to identify and characterize human fetal OECs in culture before transplantation. Anat Rec, 2010. © 2009 Wiley‐Liss, Inc.
影响因子：
6.2
作者：
Lopez-Vales, Ruben;Fores, Joaquim;Verdu, Enrique
通讯作者：
Verdu, Enrique
影响因子：
2.9
作者：
Y. Takamori;Tetsuji Mori;T. Wakabayashi;Yosuke Nagasaka;T. Matsuzaki;H. Yamada
通讯作者：
Y. Takamori;Tetsuji Mori;T. Wakabayashi;Yosuke Nagasaka;T. Matsuzaki;H. Yamada