Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds with tunable degradation and mechanical properties.
Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds with tunable degradation and mechanical properties.
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DOI：
10.1021/bm100137q
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发表时间：
2010-05-10
影响因子：
6.2
通讯作者：
Leach, Jennie B.
中科院分区：
文献类型：
--
作者：
Zustiak, Silviya P.;Leach, Jennie B.
这项工作的目的是创建三维（3D）水凝胶基质具有明确的机械性能，以及可调的降解性，用于涉及蛋白质递送和细胞封装的应用。因此，我们报告了一种新型的可水解降解的聚（乙二醇）（PEG）水凝胶的合成和表征组成的PEG乙烯基砜（PEG-VS）与PEG-二酯-二硫醇交联。与先前报道的可降解PEG基水凝胶不同，这些材料结构均匀，完全亲水，并具有高度特异性的交联化学。我们表征了水凝胶降解和机械性能的相关趋势，即，储能模量（G ′）、溶胀比（QM）和筛目尺寸（M1）。水凝胶的降解时间和监测的机械性能与交联剂分子量、交联剂官能度和总聚合物密度相关;随着降解的进行，这些性能可预测地变化（G ′降低，而QM和QM增加），直到凝胶达到完全降解。还验证了Balb/3T3成纤维细胞在3D水凝胶基质内的粘附和增殖。总之，这些独特的性质表明，所报道的可降解PEG水凝胶很好地准备用于蛋白质和细胞递送以修复软组织的特定应用。
The objective of this work was to create three-dimensional (3D) hydrogel matrices with defined mechanical properties, as well as tunable degradability for use in applications involving protein delivery and cell encapsulation. Thus, we report the synthesis and characterization of a novel hydrolytically degradable poly(ethylene glycol) (PEG) hydrogel composed of PEG vinyl sulfone (PEG-VS) cross-linked with PEG-diester-dithiol. Unlike previously reported degradable PEG-based hydrogels, these materials are homogeneous in structure, fully hydrophilic and have highly specific cross-linking chemistry. We characterized hydrogel degradation and associated trends in mechanical properties, i.e., storage modulus (G′), swelling ratio (QM), and mesh size (ξ). Degradation time and the monitored mechanical properties of the hydrogel correlated with cross-linker molecular weight, cross-linker functionality, and total polymer density; these properties changed predictably as degradation proceeded (G′ decreased, whereas QM and ξ increased) until the gels reached complete degradation. Balb/3T3 fibroblast adhesion and proliferation within the 3D hydrogel matrices were also verified. In sum, these unique properties indicate that the reported degradable PEG hydrogels are well poised for specific applications in protein and cell delivery to repair soft tissue.
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影响因子：
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