Borrelia burgdorferi genes, bb0639-0642, encode a putative putrescine/spermidine transport system, PotABCD, that is spermidine specific and essential for cell survival.
Borrelia burgdorferi genes, bb0639-0642, encode a putative putrescine/spermidine transport system, PotABCD, that is spermidine specific and essential for cell survival.
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DOI：
10.1111/mmi.13940
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发表时间：
2018-05
影响因子：
3.6
通讯作者：
Gherardini FC
中科院分区：
文献类型：
--
作者：
Bontemps-Gallo S;Lawrence KA;Richards CL;Gherardini FC
多胺是在生命的所有王国中发现的代谢物的基本类别。伯氏疏螺旋体没有合成或降解多胺的酶，但含有多胺摄取系统potABCD。在这份报告中，我们描述了这个假定的运输系统的初步表征。在几次不成功的尝试后，我们将操纵子置于诱导型LacI启动子表达系统的控制下。对该构建体的分析证实potABCD是体外存活所需的。此外，我们证明了potABCD操纵子在体外被低渗透压上调。以前，我们已经表明，低渗透压触发Rrp 2/RpoN/RpoS调节级联的激活，其调节螺旋体从蜱到哺乳动物宿主的传播所必需的基因。有趣的是，pot操纵子的诱导仅在rpoS突变体中受影响，而在rpoN突变体中不受影响，这表明基因是RpoS依赖的，RpoN独立的。此外，potABCD上调蜱喂养伴随着螺旋体复制的开始。最后，摄取实验确定了B的特异性。亚精胺的伯氏PotABCD。莱姆病病原体伯氏疏螺旋体（Borrelia burgdorferi）不具有合成或降解多胺的酶，但含有编码推定的多胺摄取系统（potABCD）的基因。在这里，我们证明了PotABCD是一种对生存至关重要的精脒特异性转运系统。这些基因在蜱虫进食期间上调，同时伴有渗透压降低，这已被证明可触发毒力因子表达。potABCD基因以RpoN依赖性、RpoS独立性、BosR独立性的方式调节。
Polyamines are an essential class of metabolites found throughout all kingdoms in life. Borrelia burgdorferi harbors no enzymes to synthesize or degrade polyamines yet does contain a polyamine uptake system, potABCD. In this report, we describe the initial characterization of this putative transport system. After several unsuccessful attempts to inactivate potABCD, we placed the operon under the control of an inducible LacI promoter expression system. Analyses of this construct confirmed that potABCD was required for in vitro survival. Additionally, we demonstrated that the potABCD operon were upregulated in vitro by low osmolarity. Previously, we had shown that low osmolarity triggers the activation of the Rrp2/RpoN/RpoS regulatory cascade which regulates genes essential for the transmission of spirochetes from ticks to mammalian hosts. Interestingly, induction of the pot operon was only affected in an rpoS mutant but not in a rpoN mutant, suggesting that the genes were RpoS-dependent, RpoN-independent. Furthermore, potABCD was upregulated during tick feeding concomitant with the initiation of spirochete replication. Finally, uptake experiments determined the specificity of B. burgdorferi’s PotABCD for spermidine. Borrelia burgdorferi, Lyme disease agent, harbors no enzymes to synthesize or degrade polyamines yet does contain the genes encoding a putative polyamine uptake system (potABCD). Here, we demonstrated that the PotABCD is a spermidine-specific transporter system that is essential for survival. The genes are upregulated during tick feeding concomitantly with the decrease of osmoliarity which has been shown to trigger virulence factor expression. The potABCD genes are regulated in a RpoN-dependent, RpoS-independent, BosR-independent fashion.
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DOI：
10.1073/pnas.0408536102
发表时间：
2005-04-05
影响因子：
11.1
作者：
Fisher, MA;Grimm, D;Gherardini, FC
通讯作者：
Gherardini, FC
DOI：
10.1073/pnas.231442498
发表时间：
2001-10-23
影响因子：
11.1
作者：
Hübner, A;Yang, XF;Norgard, MV
通讯作者：
Norgard, MV
影响因子：
3.6
作者：
Hyde JA;Shaw DK;Smith Iii R;Trzeciakowski JP;Skare JT
通讯作者：
Skare JT
影响因子：
14.9
作者：
Adams PP;Flores Avile C;Popitsch N;Bilusic I;Schroeder R;Lybecker M;Jewett MW
通讯作者：
Jewett MW
影响因子：
3.4
作者：
Caimano MJ;Drecktrah D;Kung F;Samuels DS
通讯作者：
Samuels DS