Electrophysiological and contractile function of cardiomyocytes derived from human embryonic stem cells.
Electrophysiological and contractile function of cardiomyocytes derived from human embryonic stem cells.
复制标题
DOI：
10.1016/j.pbiomolbio.2012.07.012
复制
发表时间：
2012-10
影响因子：
3.8
通讯作者：
Tung L
中科院分区：
文献类型：
--
作者：
Blazeski A;Zhu R;Hunter DW;Weinberg SH;Boheler KR;Zambidis ET;Tung L
人类胚胎干细胞已成为心肌细胞的原型来源，可用于药物发现和细胞治疗。然而，这样的应用要求这些心肌细胞（hESC-CM）忠实地再现成体细胞的生理学，特别是与它们的电生理和收缩功能有关的生理学。我们回顾什么是已知的hESC-CM的电生理学方面的跳动率，动作电位特性，离子电流和细胞耦合，以及它们的收缩性方面的钙循环和收缩。我们还讨论了在心脏分化，成熟和培养条件的变化所产生的细胞表型的异质性，并总结了目前的策略，已实施，以减少这种异质性。最后，我们提出了原始的电生理数据从hESC-CM集群的光学地图。
Human embryonic stem cells have emerged as the prototypical source from which cardiomyocytes can be derived for use in drug discovery and cell therapy. However, such applications require that these cardiomyocytes (hESC-CMs) faithfully recapitulate the physiology of adult cells, especially in relation to their electrophysiological and contractile function. We review what is known about the electrophysiology of hESC-CMs in terms of beating rate, action potential characteristics, ionic currents, and cellular coupling as well as their contractility in terms of calcium cycling and contraction. We also discuss the heterogeneity in cellular phenotypes that arises from variability in cardiac differentiation, maturation, and culture conditions, and summarize present strategies that have been implemented to reduce this heterogeneity. Finally, we present original electrophysiological data from optical maps of hESC-CM clusters.
登录
查看更多内容
影响因子：
14
作者：
Aubin H;Nichol JW;Hutson CB;Bae H;Sieminski AL;Cropek DM;Akhyari P;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
23.9
作者：
Burridge, Paul W.;Keller, Gordon;Gold, Joseph D.;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
--
作者：
Bray, Mark-Anthony;Sheehy, Sean P.;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
2.8
作者：
Awasthi, Samir;Matthews, Dennis L.;Li, Ronald A.;Chiamvimonvat, Nipavan;Lieu, Deborah K.;Chan, James W.
通讯作者：
Chan, James W.
影响因子：
3.7
作者：
Bilchick, Kenneth C;Helm, Robert H;Kass, David A
通讯作者：
Kass, David A