Label-free identification and characterization of human pluripotent stem cell-derived cardiomyocytes using second harmonic generation (SHG) microscopy.
Label-free identification and characterization of human pluripotent stem cell-derived cardiomyocytes using second harmonic generation (SHG) microscopy.
复制标题
DOI：
10.1002/jbio.201100077
复制
发表时间：
2012-01
影响因子：
2.8
通讯作者：
Chan, James W.
中科院分区：
文献类型：
--
作者：
Awasthi, Samir;Matthews, Dennis L.;Li, Ronald A.;Chiamvimonvat, Nipavan;Lieu, Deborah K.;Chan, James W.
多能干细胞来源的心肌细胞(PSC-CMS)是心脏移植治疗中潜在的无限来源的心肌细胞(CMS)。建立纯PSCCM种群对这一应用很重要，但由于缺乏适合其鉴定和分类的CM特异性表面标记，这一点受到了阻碍。当代的纯化技术要么是非特异性的，要么需要基因改造。我们报告了在PSC-CMS中可检测到的二次谐波产生(SHG)信号，该信号可归因于肌球蛋白，依赖于PSC-CM成熟度，并在PSCCMS处于悬浮状态时保留。我们的研究证明了开发SHG激活的流式细胞仪用于PSC-CMS非侵入性纯化的可行性。肌球蛋白的二次谐波产生(SHG)可用于鉴定悬液中的人多能干细胞来源的心肌细胞(PSC-CMS)，并根据成熟度区分PSC-CMS组。
Pluripotent stem cell-derived cardiomyocytes (PSC-CMs) are a potentially unlimited source of cardiomyocytes (CMs) for cardiac transplantation therapies. The establishment of pure PSCCM populations is important for this application, but is hampered by a lack of CM-specific surface markers suitable for their identification and sorting. Contemporary purification techniques are either non-specific or require genetic modification. We report a second harmonic generation (SHG) signal detectable in PSC-CMs that is attributable to sarcomeric myosin, dependent on PSC-CM maturity, and retained while PSCCMs are in suspension. Our study demonstrates the feasibility of developing a SHG-activated flow cytometer for the noninvasive purification of PSC-CMs. Second harmonic generation (SHG) from sarcomeric myosin can be used to identify human plutipotent stem cell-derived cardiomyocytes (PSC-CMs) in suspension and discriminate groups of PSC-CMs according to maturity.
登录
查看更多内容
影响因子：
2.9
作者：
Kim, Yoon Young;Ku, Seung-Yup;Choi, Young Min
通讯作者：
Choi, Young Min
影响因子：
3.4
作者：
Plotnikov, SV;Millard, AC;Mohler, WA
通讯作者：
Mohler, WA
影响因子：
2.3
作者：
ANDREWS, DL;ALLCOCK, P;DEMIDOV, AA
通讯作者：
DEMIDOV, AA
影响因子：
5
作者：
PERISSEL, B;CHARBONNE, F;MALET, P
通讯作者：
MALET, P
DOI：
10.1073/pnas.0731953100
发表时间：
2003-06-10
影响因子：
11.1
作者：
Dombeck, DA;Kasischke, KA;Webb, WW
通讯作者：
Webb, WW