Directed 3D cell alignment and elongation in microengineered hydrogels.
Directed 3D cell alignment and elongation in microengineered hydrogels.
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DOI：
10.1016/j.biomaterials.2010.05.056
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发表时间：
2010-09
期刊：
影响因子：
14
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Aubin H;Nichol JW;Hutson CB;Bae H;Sieminski AL;Cropek DM;Akhyari P;Khademhosseini A
有组织的细胞排列是控制组织微结构和生物功能的关键。虽然已经描述了许多技术来控制2D中的细胞排列，但在3D工程组织中重述高度组织的自然组织的细胞排列仍然是一个挑战。虽然工程组织中的细胞排列可以通过使用外部物理刺激来诱导，但很少有简单的技术可以在很大程度上由细胞驱动的微尺度控制细胞行为。在这项研究中，我们提出了一种简单而直接的方法来控制包裹在微工程3D明胶甲基丙烯酸酯(GelMA)水凝胶中的成纤维细胞、成肌细胞、内皮细胞和心脏干细胞的排列和伸长，表明如果限制在适当的3D微结构中，具有在体内形成排列组织的内在潜力的细胞将在体外自组织形成功能组织。该系统可作为研究三维细胞和组织形态发生的体外模型，以及创建具有三维细胞排列和伸长的微尺度控制的组织结构，这可能在具有排列的细胞和各向异性功能的功能组织工程中具有巨大的潜力。
Organized cellular alignment is critical to controlling tissue microarchitecture and biological function. Although a multitude of techniques have been described to control cellular alignment in 2D, recapitulating the cellular alignment of highly organized native tissues in 3D engineered tissues remains a challenge. While cellular alignment in engineered tissues can be induced through the use of external physical stimuli, there are few simple techniques for microscale control of cell behavior that are largely cell-driven. In this study we present a simple and direct method to control the alignment and elongation of fibroblasts, myoblasts, endothelial cells and cardiac stem cells encapsulated in microengineered 3D gelatin methacrylated (GelMA) hydrogels, demonstrating that cells with the intrinsic potential to form aligned tissues in vivo will self-organize into functional tissues in vitro if confined in the appropriate 3D microarchitecture. The presented system may be used as an in vitro model for investigating cell and tissue morphogenesis in 3D, as well as for creating tissue constructs with microscale control of 3D cellular alignment and elongation, that could have great potential for the engineering of functional tissues with aligned cells and anisotropic function.
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影响因子：
14
作者：
Charest, JL;Eliason, MT;King, WP
通讯作者：
King, WP
DOI：
10.1073/pnas.0801866105
发表时间：
2008-07-15
影响因子：
11.1
作者：
Du, Yanan;Lo, Edward;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
41.2
作者：
Luo, Y;Shoichet, MS
通讯作者：
Shoichet, MS
DOI：
10.1073/pnas.94.3.849
发表时间：
1997-02-04
影响因子：
11.1
作者：
Maniotis, AJ;Chen, CS;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
14
作者：
Lee, Soo-Hong;Moon, James J.;West, Jennifer L.
通讯作者：
West, Jennifer L.