Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus.
Motilin Stimulates Gastric Acid Secretion in Coordination with Ghrelin in Suncus murinus.
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DOI：
10.1371/journal.pone.0131554
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Sakata I
中科院分区：
文献类型：
--
作者：
Goswami C;Shimada Y;Yoshimura M;Mondal A;Oda S;Tanaka T;Sakai T;Sakata I
胃动素和胃饥饿素组成了一个肽家族，这些激素对调节胃肠运动非常重要。在这项研究中，我们研究了胃动素和胃促生长素对胃酸分泌的影响在麻醉的臭鼩（家麝香鼩，臭鼩），胃促生长素和胃动素生产的哺乳动物。我们首先建立了一个胃腔灌流系统，并证实静脉（i. v.）给予组胺（1 mg/kg体重）刺激酸分泌。胃动素（0.1、1.0和10 μg/kg BW）以剂量依赖性方式刺激悬铃木的酸分泌，而单独的ghrelin（0.1、1.0和10 μg/kg BW）不诱导酸分泌。此外，与溶剂给药相比，低剂量（1 μg/kg BW）胃动素和生长素释放肽联合给药显著刺激胃酸分泌，而胃动素（1 μg/kg BW）或生长素释放肽（1 μg/kg BW）单独给药均不显著诱导胃酸分泌。这表明胃促生长素在胃动素诱导的胃酸分泌中的附加作用。然后，我们研究了胃动素/胃动素和生长激素释放肽刺激的酸分泌，使用受体拮抗剂的途径。用YM 022（一种CCK-B受体拮抗剂）和阿托品（一种毒蕈碱乙酰胆碱受体拮抗剂）处理对胃动素或胃动素-生长素释放肽联合给药诱导的酸分泌没有影响。与此相反，法莫替丁（组胺H2受体拮抗剂）完全抑制胃动素刺激的胃酸分泌和胃动素和胃饥饿素共同给药诱导的胃酸分泌。这是首次报道胃动素刺激哺乳动物胃分泌。我们的研究结果还表明，胃动素和胃动素和生长激素释放肽的共同管理刺激胃酸分泌通过组胺介导的途径在向日葵。
Motilin and ghrelin constitute a peptide family, and these hormones are important for the regulation of gastrointestinal motility. In this study, we examined the effect of motilin and ghrelin on gastric acid secretion in anesthetized suncus (house musk shrew, Suncus murinus), a ghrelin- and motilin-producing mammal. We first established a gastric lumen-perfusion system in the suncus and confirmed that intravenous (i.v.) administration of histamine (1 mg/kg body weight) stimulated acid secretion. Motilin (0.1, 1.0, and 10 μg/kg BW) stimulated the acid output in a dose-dependent manner in suncus, whereas ghrelin (0.1, 1.0, and 10 μg/kg BW) alone did not induce acid output. Furthermore, in comparison with the vehicle administration, the co-administration of low-dose (1 μg/kg BW) motilin and ghrelin significantly stimulated gastric acid secretion, whereas either motilin (1 μg/kg BW) or ghrelin (1 μg/kg BW) alone did not significantly induce gastric acid secretion. This indicates an additive role of ghrelin in motilin-induced gastric acid secretion. We then investigated the pathways of motilin/motilin and ghrelin-stimulated acid secretion using receptor antagonists. Treatment with YM 022 (a CCK-B receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist) had no effect on motilin or motilin-ghrelin co-administration-induced acid output. In contrast, famotidine (a histamine H2 receptor antagonist) completely inhibited motilin-stimulated acid secretion and co-administration of motilin and ghrelin induced gastric acid output. This is the first report demonstrating that motilin stimulates gastric secretion in mammals. Our results also suggest that motilin and co-administration of motilin and ghrelin stimulate gastric acid secretion via the histamine-mediated pathway in suncus.
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影响因子：
3
作者：
Miura, Tohru;Maruyama, Keisuke;Matsuda, Kouhei
通讯作者：
Matsuda, Kouhei
DOI：
10.1139/o73-066
发表时间：
1973-01-01
期刊：
CANADIAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BROWN, JC;COOK, MA;DRYBURGH, JR
通讯作者：
DRYBURGH, JR
影响因子：
3.6
作者：
Javid, FA;Naylor, RJ
通讯作者：
Naylor, RJ
影响因子：
--
作者：
MATSUNAGA, Y;YAMAMOTO, O;ITOH, Z
通讯作者：
ITOH, Z
DOI：
10.1254/jjp.55.425
发表时间：
1991-04-01
期刊：
JAPANESE JOURNAL OF PHARMACOLOGY
影响因子：
--
作者：
NAGAYA, H;INATOMI, N;SATOH, H
通讯作者：
SATOH, H