MR1-Restricted T Cells with MAIT-like Characteristics Are Functionally Conserved in the Pteropid Bat Pteropus alecto.
MR1-Restricted T Cells with MAIT-like Characteristics Are Functionally Conserved in the Pteropid Bat Pteropus alecto.
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DOI：
10.1016/j.isci.2020.101876
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发表时间：
2020-12-18
期刊：
影响因子：
5.8
通讯作者：
Wang LF
中科院分区：
文献类型：
--
作者：
Leeansyah E;Hey YY;Sia WR;Ng JHJ;Gulam MY;Boulouis C;Zhu F;Ahn M;Mak JYW;Fairlie DP;Kwa ALH;Sandberg JK;Wang LF
蝙蝠是大量病毒的宿主，这些病毒有可能导致重大人类疾病爆发，包括目前的2019年冠状病毒病（COVID-19）大流行。人们正在努力了解蝙蝠对病毒的免疫反应，而对它们对细菌的免疫反应知之甚少。在这项研究中，MR 1-限制性T（MR 1 T）细胞检测通过使用MR 1四聚体在循环和组织的Pteropus alecto（Pa）蝙蝠。Pa MR 1 T细胞在静息状态下对MR 1呈递的微生物代谢产物表现出弱反应。然而，与MR 1呈现激动剂引发后，他们增殖，上调关键转录因子和细胞溶解蛋白，并获得瞬时表达的Th 1/17相关的细胞因子和抗菌细胞毒性。总的来说，这些研究结果表明，蝙蝠免疫系统包括一个丰富的和功能保守的群体MR 1 T细胞与粘膜相关的不变的T样特征，这表明MR 1和MR 1 T细胞也发挥了重要作用，蝙蝠免疫防御。MR 1 T细胞存在于Pa蝙蝠中并与MR 1呈递的微生物代谢物反应Pa MR 1 T细胞在与MR 1激动剂一起培养时上调Prf和MAIT相关TF在刺激后，Pa MR 1 T细胞快速且瞬时表达TNF和IL-17 Pa MR 1 T细胞以MR 1依赖性方式杀死大肠杆菌和MR 1激动剂脉冲的细胞生物科学;免疫学;免疫系统的组成部分
Bats are reservoirs for a large number of viruses which have potential to cause major human disease outbreaks, including the current coronavirus disease 2019 (COVID-19) pandemic. Major efforts are underway to understand bat immune response to viruses, whereas much less is known about their immune responses to bacteria. In this study, MR1-restricted T (MR1T) cells were detected through the use of MR1 tetramers in circulation and tissues of Pteropus alecto (Pa) bats. Pa MR1T cells exhibited weak responses to MR1-presented microbial metabolites at resting state. However, following priming with MR1-presented agonist they proliferated, upregulated critical transcription factors and cytolytic proteins, and gained transient expression of Th1/17-related cytokines and antibacterial cytotoxicity. Collectively, these findings show that the Pa bat immune system encompasses an abundant and functionally conserved population of MR1T cells with mucosal-associated invariant T-like characteristics, suggesting that MR1 and MR1T cells also play a significant role in bat immune defense. MR1T cells are present in Pa bats and react to MR1-presented microbial metabolites Pa MR1T cells upregulate Prf and MAIT-associated TFs upon culture with MR1 agonists Upon stimulation, Pa MR1T cells rapidly and transiently express TNF and IL-17 Pa MR1T cells kill E. coli and MR1 agonist-pulsed cells in an MR1-dependent manner Biological Sciences; Immunology; Components of the Immune Systems
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影响因子：
8
作者：
Gibbs A;Leeansyah E;Introini A;Paquin-Proulx D;Hasselrot K;Andersson E;Broliden K;Sandberg JK;Tjernlund A
通讯作者：
Tjernlund A
DOI：
10.1126/science.aax6624
发表时间：
2019-10-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Constantinides MG;Link VM;Tamoutounour S;Wong AC;Perez-Chaparro PJ;Han SJ;Chen YE;Li K;Farhat S;Weckel A;Krishnamurthy SR;Vujkovic-Cvijin I;Linehan JL;Bouladoux N;Merrill ED;Roy S;Cua DJ;Adams EJ;Bhandoola A;Scharschmidt TC;Aubé J;Fischbach MA;Belkaid Y
通讯作者：
Belkaid Y
影响因子：
9.8
作者：
Boulouis, Caroline;Sia, Wan Rong;Leeansyah, Edwin
通讯作者：
Leeansyah, Edwin
影响因子：
3.7
作者：
Claudio, Vinicius C.;Gonzalez, Irys;Rassy, Fabricio
通讯作者：
Rassy, Fabricio
影响因子：
3.7
作者：
Gutiérrez-Preciado A;Torres AG;Merino E;Bonomi HR;Goldbaum FA;García-Angulo VA
通讯作者：
García-Angulo VA