Probing the stochastic, motor-driven properties of the cytoplasm using force spectrum microscopy.
Probing the stochastic, motor-driven properties of the cytoplasm using force spectrum microscopy.
复制标题
DOI：
10.1016/j.cell.2014.06.051
复制
发表时间：
2014-08-14
期刊：
影响因子：
64.5
通讯作者：
Weitz DA
中科院分区：
文献类型：
--
作者：
Guo M;Ehrlicher AJ;Jensen MH;Renz M;Moore JR;Goldman RD;Lippincott-Schwartz J;Mackintosh FC;Weitz DA
细胞中的分子马达通常会产生高度定向的运动;然而，所有活动过程的聚集、不相干效应也会产生随机波动的力，这些力会驱动类似扩散的非热运动。在这里，我们引入力谱显微镜（FSM）直接量化细胞质内的随机力，从而探测随机运动活动。这种技术结合了测量探针粒子的随机运动与独立的细胞质的微机械测量，以量化力的波动谱。使用有限状态机，我们表明，力的波动大大提高细胞内的运动的小型和大型组件。恶性细胞中的波动比良性细胞大三倍。我们进一步证明，波形蛋白的行为全球锚细胞器对随机波动的力量在细胞质中，其大小没有影响。因此，有限状态机有广泛的应用，了解细胞质和细胞内的过程，在健康和疾病状态下的细胞生理学。
Molecular motors in cells typically produce highly directed motion; however, the aggregate, incoherent effect of all active processes also creates randomly fluctuating forces, which drive diffusive-like, non-thermal motion. Here we introduce force-spectrum-microscopy (FSM) to directly quantify random forces within the cytoplasm of cells and thereby probe stochastic motor activity. This technique combines measurements of the random motion of probe particles with independent micromechanical measurements of the cytoplasm to quantify the spectrum of force fluctuations. Using FSM, we show that force fluctuations substantially enhance intracellular movement of small and large components. The fluctuations are three times larger in malignant cells than in their benign counterparts. We further demonstrate that vimentin acts globally to anchor organelles against randomly fluctuating forces in the cytoplasm, with no effect on their magnitude. Thus, FSM has broad applications for understanding the cytoplasm and its intracellular processes in relation to cell physiology in healthy and diseased states.
登录
查看更多内容
细胞质扩散：分子电机将其混合。
影响因子：
7.8
作者：
Brangwynne, Clifford P.;Koenderink, Gijsje H.;MacKintosh, Frederick C.;Weitz, David A.
通讯作者：
Weitz, David A.
DOI：
10.1073/pnas.1215462109
发表时间：
2012-11-06
影响因子：
11.1
作者：
Hendricks, Adam G.;Holzbaur, Erika L. F.;Goldman, Yale E.
通讯作者：
Goldman, Yale E.
影响因子：
64.5
作者：
Ananthanarayanan, Vaishnavi;Schattat, Martin;Tolic-Norrelykke, Iva M.
通讯作者：
Tolic-Norrelykke, Iva M.
影响因子：
3.7
作者：
Hale, Christopher M.;Sun, Sean X.;Wirtz, Denis
通讯作者：
Wirtz, Denis
影响因子：
4.6
作者：
Agus, David B.;Alexander, Jenolyn F.;Arap, Wadih;Ashili, Shashanka;Aslan, Joseph E.;Austin, Robert H.;Backman, Vadim;Bethel, Kelly J.;Bonneau, Richard;Chen, Wei-Chiang;Chen-Tanyolac, Chira;Choi, Nathan C.;Curley, Steven A.;Dallas, Matthew;Damania, Dhwanil;Davies, Paul C. W.;Decuzzi, Paolo;Dickinson, Laura;Estevez-Salmeron, Luis;Estrella, Veronica;Ferrari, Mauro;Fischbach, Claudia;Foo, Jasmine;Fraley, Stephanie I.;Frantz, Christian;Fuhrmann, Alexander;Gascard, Philippe;Gatenby, Robert A.;Geng, Yue;Gerecht, Sharon;Gillies, Robert J.;Godin, Biana;Grady, William M.;Greenfield, Alex;Hemphill, Courtney;Hempstead, Barbara L.;Hielscher, Abigail;Hillis, W. Daniel;Holland, Eric C.;Ibrahim-Hashim, Arig;Jacks, Tyler;Johnson, Roger H.;Joo, Ahyoung;Katz, Jonathan E.;Kelbauskas, Laimonas;Kesselman, Carl;King, Michael R.;Konstantopoulos, Konstantinos;Kraning-Rush, Casey M.;Kuhn, Peter;Kung, Kevin;Kwee, Brian;Lakins, Johnathon N.;Lambert, Guillaume;Liao, David;Licht, Jonathan D.;Liphardt, Jan T.;Liu, Liyu;Lloyd, Mark C.;Lyubimova, Anna;Mallick, Parag;Marko, John;McCarty, Owen J. T.;Meldrum, Deirdre R.;Michor, Franziska;Mumenthaler, Shannon M.;Nandakumar, Vivek;O'Halloran, Thomas V.;Oh, Steve;Pasqualini, Renata;Paszek, Matthew J.;Philips, Kevin G.;Poultney, Christopher S.;Rana, Kuldeepsinh;Reinhart-King, Cynthia A.;Ros, Robert;Semenza, Gregg L.;Senechal, Patti;Shuler, Michael L.;Srinivasan, Srimeenakshi;Staunton, Jack R.;Stypula, Yolanda;Subramanian, Hariharan;Tlsty, Thea D.;Tormoen, Garth W.;Tseng, Yiider;van Oudenaarden, Alexander;Verbridge, Scott S.;Wan, Jenny C.;Weaver, Valerie M.;Widom, Jonathan;Will, Christine;Wirtz, Denis;Wojtkowiak, Jonathan;Wu, Pei-Hsun
通讯作者：
Wu, Pei-Hsun